Yazdiniapour Zeinab, Mirian Mina, Zolfaghari Behzad, Mehdifar Pegah, Ghanadian Mustafa, Lanzotti Virginia
Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran; Department of Pharmacognosy, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Pharmaceutical Biotechnology, Isfahan University of Medical Sciences, Isfahan, Iran.
Fitoterapia. 2022 Mar;157:105138. doi: 10.1016/j.fitote.2022.105138. Epub 2022 Jan 31.
Phytochemical analysis of Euphorbia gedrosiaca Rech.f., Aellen & Esfand., an Iranian endemic spurge, afforded the isolation of four myrsinane types diterpene polyesters. Two new compounds (1-2) were based on a myrsinane skeleton while the others (3-4) were known diterpenes based on a cyclomyrsinane backbone. Their chemical structures were elucidated by spectroscopic methods, including 1D and 2D NMR and HRESIMS. The isolated compounds were tested to evaluate their cell growth inhibitory activity and apoptotic effects on melanoma cell lines, B16F10 and A375. The IC values for compounds 1-4 were 58.45, 55.43, 86.52 and 82.27 μM, respectively, on B16F10, and 20.66, 21.88, 36.21 and 39.87 μM, respectively, on A375 cells. Non-treated cells were used as negative control (100% cell growth) and 5 nM Taxol were considered as a positive control.
对伊朗特有大戟属植物格德罗西亚大戟（Euphorbia gedrosiaca Rech.f., Aellen & Esfand.）进行植物化学分析，分离出了四种海桐烷型二萜聚酯。两种新化合物（1 - 2）基于海桐烷骨架，而其他化合物（3 - 4）是基于环海桐烷骨架的已知二萜。通过包括一维和二维核磁共振以及高分辨电喷雾电离质谱在内的光谱方法阐明了它们的化学结构。对分离出的化合物进行测试，以评估它们对黑色素瘤细胞系B16F10和A375的细胞生长抑制活性和凋亡作用。化合物1 - 4对B16F10的IC值分别为58.45、55.43、86.52和82.27 μM，对A375细胞的IC值分别为20.66、21.88、36.21和39.87 μM。未处理的细胞用作阴性对照（100%细胞生长），5 nM紫杉醇被视为阳性对照。
