Yazdiniapour Zeinab, Sohrabi Mohammad Hossein, Motinia Newsha, Zolfaghari Behzad, Mehdifar Pegah, Ghanadian Mustafa, Lanzotti Virginia
Department of Pharmacognosy, Isfahan University of Medical Sciences, Isfahan 81746-73461, Iran.
Dipartimento di Agraria, Università di Napoli Federico II, 80055 Portici, Napoli, Italy.
Metabolites. 2023 Feb 3;13(2):225. doi: 10.3390/metabo13020225.
Isolated diterpenes from various species of are important compounds for drug discovery with a broad spectrum of structures and biological effects. In this study, , one of the endemic species of Iran, was analyzed in terms of the presence and structural determination of diterpenoid compounds. They were extracted with dichloromethane/acetone (2:1) from aerial parts of this plant and purified by chromatographic methods such as MPLC and HPLC. Four premyrsinane compounds and one myrsinane diterpene were isolated from . They were characterized by extensive 1D and 2D NMR and HRMS analyses. Additionally, their activities were evaluated against two breast cancer cell lines, MDA-MB-231 and MCF-7, by MTT proliferation assay. They exhibited cytotoxic effects in a dose-dependent manner with promising results, which can help to find possible therapeutic application of diterpenoids in breast cancer treatment.
从各种植物中分离出的二萜类化合物是药物研发的重要化合物，具有广泛的结构和生物效应。在本研究中，对伊朗特有物种之一的[植物名称]进行了二萜类化合物的存在情况及结构测定分析。用二氯甲烷/丙酮（2:1）从该植物地上部分提取它们，并通过MPLC和HPLC等色谱方法进行纯化。从[植物名称]中分离出四种前迈森烷化合物和一种迈森烷二萜。通过广泛的一维和二维核磁共振以及高分辨质谱分析对它们进行了表征。此外，通过MTT增殖试验评估了它们对两种乳腺癌细胞系MDA-MB-231和MCF-7的活性。它们以剂量依赖性方式表现出细胞毒性作用，结果令人鼓舞，这有助于找到二萜类化合物在乳腺癌治疗中的潜在治疗应用。
