School of Chemistry and Life Science, Advanced Institute of Materials Science, Changchun University of Technology, 2055 Yanan Street, Changchun 130012, People's Republic of China.
School of Public Health, Nantong University, No. 9, Seyuan Road, Nantong 226019, People's Republic of China.
Nanoscale. 2022 Feb 17;14(7):2676-2685. doi: 10.1039/d1nr07629a.
The construction of multifunctional nano-enzymes is a feasible strategy for fighting multi-drug resistant (MDR) bacterial biofilm-associated infections. Extracellular DNA (eDNA) is an important functional part of biofilm formation, including the initial adherence of bacteria to subsequent development and eventual maturation. A nano-enzyme platform of graphene oxide-based nitrilotriacetic acid-cerium(IV) composite (GO-NTA-Ce) against bacterial biofilm infection has been developed. When located at the site of bacteria-associated infection, GO-NTA-Ce could inhibit the biofilm formation and effectively disperse the formed biofilm by degrading the eDNA. In addition to Ce-mediated deoxyribonuclease (DNase)-like activity, near-infrared laser irradiation of GO-NTA-Ce could produce local hyperthermia to kill the bacteria that lost the protection by the biofilm matrix. In addition, graphene is also a new green broad-spectrum antimicrobial material that can exert its antimicrobial effects through physical damage and chemical damage. In short, our GO-NTA-Ce nano-enzyme platform is capable of effectively eradicating drug-resistant bacterial biofilm infections through the triple action of DNase-like enzyme properties, photothermal therapy, and graphene-based antimicrobial activity, and the nano-composite has excellent potential for the treatment of MDR bacterial biofilm infections.
构建多功能纳米酶是对抗多药耐药（MDR）细菌生物膜相关感染的可行策略。细胞外 DNA（eDNA）是生物膜形成的一个重要功能部分，包括细菌最初的黏附到随后的发展和最终的成熟。开发了一种基于氧化石墨烯的氮三乙酸-铈（IV）复合纳米酶平台（GO-NTA-Ce）来对抗细菌生物膜感染。当位于与细菌相关的感染部位时，GO-NTA-Ce 可以通过降解 eDNA 来抑制生物膜的形成并有效分散已形成的生物膜。除了 Ce 介导的脱氧核糖核酸酶（DNase）样活性外，GO-NTA-Ce 的近红外激光照射还可以产生局部热疗，杀死失去生物膜基质保护的细菌。此外，石墨烯也是一种新型的绿色广谱抗菌材料，可通过物理损伤和化学损伤发挥其抗菌作用。总之，我们的 GO-NTA-Ce 纳米酶平台能够通过 DNase 样酶特性、光热疗法和基于石墨烯的抗菌活性的三重作用，有效根除耐药细菌生物膜感染，该纳米复合材料在治疗 MDR 细菌生物膜感染方面具有巨大的潜力。
