Fan Weiguang, Su Miaomiao, Meng Juan, Yang Xuegang, Liu Zhenxia, Wang Hao, Shi Haoxi, Lu Xinli, Shi Penghui
Department of Clinical Laboratory Medicine, The People's Hospital of Baoding, Baoding, China.
Department of Infection Division, The People's Hospital of Baoding, Baoding, China.
AIDS Res Hum Retroviruses. 2022 Mar;38(3):237-241. doi: 10.1089/AID.2021.0209.
We analyzed the near full-length genome (NFLG) of an HIV-1-positive sample(027A) with an undetermined subtype to determine the recombinant characteristics and possible source of the parental virus. 027A is a novel HIV-1 second-generation recombinant form composed of CRF01_AE and subtype B, detected from a married HIV-1-positive male subject who was infected through homosexual transmission in Baoding, Hebei province, China. The NFLG phylogenetic tree analysis suggested that the strain was close to circulation recombinant forms' (CRFs') reference sequences involved with CRF01_AE, but formed a distinct monophyletic cluster separately from them. This indicated that the strain might be a unique CRF01_AE-related recombinant from. Furthermore, the results of RIP and jpHMM further demonstrated that the NFLG sequence of the strain was composed of CRF01_AE and subtype B. The strain was two CRF01_AE fragments inserted into B backbone. Phylogenetic analysis illustrated that the CRF01_AE subregions were from the previously identified CRF01_AE cluster 4, and the B subregions were correlated with the B strains originated from Europe and America. They were all the lineages widely prevalent in men who have sex with men (MSM) population in China. In recent years, a large number of recombinant originated from CRF01_AE and B strains are constantly emerging in MSM population in China. This continual and recurrent recombination between CRF01_AE and B in high-risk group people deserves more attention and further monitoring.
我们分析了一份HIV-1阳性样本（027A）的近全长基因组（NFLG），该样本亚型未确定，以确定其重组特征和亲本病毒的可能来源。027A是一种新型的HIV-1第二代重组形式，由CRF01_AE和B亚型组成，从一名已婚的HIV-1阳性男性受试者中检测到，该受试者在中国河北省保定市通过同性传播感染。NFLG系统发育树分析表明，该毒株与涉及CRF01_AE的循环重组形式（CRFs）参考序列接近，但与它们分别形成一个独特的单系簇。这表明该毒株可能是一种独特的与CRF01_AE相关的重组毒株。此外，RIP和jpHMM的结果进一步证明，该毒株的NFLG序列由CRF01_AE和B亚型组成。该毒株是两个插入B主干的CRF01_AE片段。系统发育分析表明，CRF01_AE亚区域来自先前确定的CRF01_AE簇4，B亚区域与源自欧美地区的B毒株相关。它们都是在中国男男性行为者（MSM）人群中广泛流行的谱系。近年来，中国MSM人群中不断出现大量源自CRF01_AE和B毒株的重组毒株。CRF01_AE和B在高危人群中的这种持续和反复重组值得更多关注和进一步监测。
