Huang Le Yi, Sun Yu Zheng, Chen Qian Qian, Bai Xu Lan, Du Ting Ting, Xu Hong Tao, Chou Gui Xin
The MOE Key Laboratory of Standardization of Chinese Medicines and SATCM Key Laboratory of New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, P.R.China.
Nat Prod Res. 2023 Jul;37(13):2189-2197. doi: 10.1080/14786419.2022.2035382. Epub 2022 Feb 3.
Five new iridoids, patriscabioins M-Q and a new monoterpene, eldanolide acid, together with three known iridoids, were isolated from the 95% aqueous EtOH extract whole plants of Juss. The structures were established by a variety of spectroscopic analysis, such as IR, 1 D and 2 D NMR spectra, MS, ECD and X-ray diffraction data. Bioactivity screening revealed the inhibitory effects on nitric oxide (NO) production of them in lipopolysaccharide-activated RAW264.7 cells with Aminoguanidine Hydrochloride as the positive control. Among them, patriscabioin M (1), patriscabioin N (2), patriscabioin P (4), patriscabioin Q (5), 8,9-didehydro-7-hydroxydolichodia (7) were found to markedly reduce LPS-induced NO production in murine macrophage cells with IC values of 18.14, 18.93, 22.00, 13.64, 26.48 μM, respectively.
从菊科植物全株的95%乙醇提取物中分离得到5个新的环烯醚萜类化合物，即patriscabioins M-Q和1个新的单萜类化合物eldanolide acid，以及3个已知的环烯醚萜类化合物。通过多种光谱分析方法，如红外光谱（IR）、一维和二维核磁共振谱（1D和2D NMR）、质谱（MS）、电子圆二色谱（ECD）和X射线衍射数据确定了它们的结构。生物活性筛选显示，以盐酸氨基胍为阳性对照，它们对脂多糖激活的RAW264.7细胞中一氧化氮（NO）的产生具有抑制作用。其中，patriscabioin M（1）、patriscabioin N（2）、patriscabioin P（4）、patriscabioin Q（5）、8,9-二脱氢-7-羟基多枝鼠尾草素（7）被发现能显著降低小鼠巨噬细胞中脂多糖诱导的NO产生，其IC50值分别为18.14、18.93、22.00、13.64、26.48 μM。
