Liu Zhi-Yuan, Guo Sheng-Run, Tang Jian-Xian, Zhao Chun-Yan, Pu Li, Wang Li-Zhong, Liu Dan, Li Rong-Tao, Li Hong-Mei
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, Yunnan, PR China.
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, Yunnan, PR China.
Phytochemistry. 2025 Nov;239:114609. doi: 10.1016/j.phytochem.2025.114609. Epub 2025 Jul 1.
Forty-three compounds, including nine undescribed compounds, five iridoids (patrirupesins A-E, 1-5) and four bis-iridoids (patrirupesins F-I, 6-9), were isolated from the EtOAc-soluble fraction of the aerial parts of Patrinia rupestris (Pall.) Dufr., and their structures were elucidated based on Mass spectrometry and NMR spectroscopy. The absolute configuration of patrirupesins A-E (1-5) and I (9) was determined by experimental and calculated electronic circular dichroism (ECD). In addition, patrirupesin E (5), patrirupesin H (8), 8,9-didehydro-7-hydroxydolichodial (17), 5,6-epoxy-3-hydroxy-7-megastigmen-9-one (19), iso-seco-tanapartholide (24) and 3-hydroxy-2-methyl-4-(3-oxoprop-1-en-2-yl)cyclopent-1-enecarbaldehyde (43) showed in vitro anti-inflammatory activity on NO production in RAW 264.7 cells stimulated by lipopolysaccharide (LPS) with IC values of 9.32 ± 3.83, 4.63 ± 1.23, 8.31 ± 4.05, 17.23 ± 5.19, 8.30 ± 6.17 and 27.38 ± 4.69 μM, respectively.
