School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Japan.
Department of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria.
J Vet Med Sci. 2022 Mar 3;84(3):338-341. doi: 10.1292/jvms.21-0636. Epub 2022 Feb 2.
The present study examined the presence of Babesia parasites in 104 domestic dogs in Nigeria. Sequentially, Babesia parasites infecting domestic dogs underwent genetic and phylogenetic analyses. The results of nested PCR based on the Piroplasmida 18S rRNA gene illustrated that 13.5% (14/104) of the samples were positive. The obtained positive samples determined the nucleotide sequences of the 18S rRNA genes. In the genetic and phylogenetic analyses, four of five nucleotide sequences were similar to Babesia canis rossi, and one sample exhibited a close similarity to a Babesia sp. isolated from a raccoon in Hokkaido, Japan. The present study revealed the widespread presence of B. canis rossi among domestic dogs in Nigeria.
本研究检测了 104 只尼日利亚国内犬中的巴贝西虫寄生虫。随后，对感染国内犬的巴贝西虫寄生虫进行了基因和系统发育分析。基于 Piroplasmida 18S rRNA 基因的巢式 PCR 的结果表明，13.5%（14/104）的样本呈阳性。获得的阳性样本确定了 18S rRNA 基因的核苷酸序列。在遗传和系统发育分析中，5 个核苷酸序列中的 4 个与犬巴贝斯虫相似，一个样本与从日本北海道浣熊中分离出的巴贝斯虫属密切相似。本研究表明，尼日利亚国内犬中广泛存在犬巴贝斯虫。
