Yi Xiaozhe, Wang Xingwen, Wu Lan, Wang Mengyue, Yang Liu, Liu Xia, Chen Shilin, Shi Yuhua
Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan, China.
Front Plant Sci. 2022 Jan 17;12:811166. doi: 10.3389/fpls.2021.811166. eCollection 2021.
is a valuable traditional medicinal plant in Asia. The essential oil from its leaves is rich in terpenoids and has been used to enhance health and well-being. In China, the market scale of industries related to has attained tens of billions of Chinese Yuan. The basic helix-loop-helix (bHLH) family is one of the largest transcription factors families in plants that plays crucial roles in diverse biological processes and is an essential regulatory component of terpenoid biosynthesis. However, the bHLH TFs and their regulatory roles in remain unknown. Here, 53 genes were identified from the transcriptome of and were classified into 15 subfamilies based on the classification of bHLH proteins in . The MEME analysis showed that the conserved motif 1 and motif 2 constituted the most conserved bHLH domain and distributed in most AarbHLH proteins. Additionally, integrated analysis of the expression profiles of genes and the contents of targeted terpenoids in different tissues group and JA-treated group were performed. Eleven up-regulated and one down-regulated were screened as candidate genes that may participate in the regulation of terpenoid biosynthesis (). Correlation analysis between gene expression and terpenoid contents indicated that the gene expression of these 12 was significantly correlated with the content changes of 1,8-cineole or β-caryophyllene. Protein-protein interaction networks further illustrated that these might be involved in terpenoid biosynthesis in . This finding provides a basis to further investigate the regulation mechanism of genes in terpenoid biosynthesis, and will be helpful to improve the quality of .
是亚洲一种珍贵的传统药用植物。其叶片中的精油富含萜类化合物，一直被用于增进健康和福祉。在中国，与之相关的产业市场规模已达数百亿元人民币。基本螺旋-环-螺旋（bHLH）家族是植物中最大的转录因子家族之一，在多种生物过程中发挥关键作用，是萜类生物合成的重要调控成分。然而，bHLH转录因子及其在[植物名称]中的调控作用仍不清楚。在此，从[植物名称]的转录组中鉴定出53个[植物名称]基因，并根据[参考植物名称]中bHLH蛋白的分类将其分为15个亚家族。MEME分析表明，保守基序1和基序2构成了最保守的bHLH结构域，分布于大多数[植物名称]bHLH蛋白中。此外，还对不同组织组和茉莉酸处理组中[植物名称]基因的表达谱和靶向萜类化合物的含量进行了综合分析。筛选出11个上调的[植物名称]基因和1个下调的[植物名称]基因作为可能参与萜类生物合成调控的候选基因（[表格名称]）。基因表达与萜类化合物含量的相关性分析表明，这12个[植物名称]基因的表达与1,8-桉叶素或β-石竹烯的含量变化显著相关。蛋白质-蛋白质相互作用网络进一步表明，这些[植物名称]基因可能参与[植物名称]中的萜类生物合成。这一发现为进一步研究[植物名称]基因在萜类生物合成中的调控机制提供了依据，并将有助于提高[植物名称]的品质。
