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R2R3-MYB转录因子NtMYB330调控烟草（Nicotiana tabacum L.）中原花青素的生物合成和种子萌发

R2R3-MYB Transcription Factor NtMYB330 Regulates Proanthocyanidin Biosynthesis and Seed Germination in Tobacco ( L.).

作者信息

Zhao Lu, Song Zhongbang, Wang Bingwu, Gao Yulong, Shi Junli, Sui Xueyi, Chen Xuejun, Zhang Yihan, Li Yongping

机构信息

Key Laboratory of Tobacco Biotechnological Breeding, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, China.

National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, China.

出版信息

Front Plant Sci. 2022 Jan 17;12:819247. doi: 10.3389/fpls.2021.819247. eCollection 2021.

DOI:10.3389/fpls.2021.819247
PMID:35111187
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8801704/
Abstract

Proanthocyanidins (PAs) are important phenolic compounds and PA biosynthesis is regulated by a ternary MBW complex consisting of a R2R3-MYB regulator, a bHLH factor and a WDR protein. In this study, a tobacco R2R3-MYB factor NtMYB330 was characterized as the PA-specific regulator in which the PA biosynthesis was promoted in the flowers of -overexpressing lines while decreased in the flowers of mutants. NtMYB330 can interact with flavonoid-related bHLH partner NtAn1b and WDR protein NtAn11-1, and the NtMYB330-NtAn1b complex is required to achieve strong transcriptional activation of the PA-related structural genes , , and . Our data reveal that NtMYB330 regulates PA biosynthesis in seeds and affects seed germination, in which -overexpressing lines showed higher PA accumulations in seed coats and inhibited germination, while mutants had reduced seed coat PAs and improved germination. NtMYB330 affects seed germination possibly through two mechanisms: modulating seed coat PAs to affect coat-imposed dormancy. In addition, NtMYB330 regulates the expressions of abscisic acid (ABA) and gibberellin acid (GA) signaling-related genes, affecting ABA-GA crosstalk and seed germination. This study reveals that NtMYB330 specifically regulates PA biosynthesis via formation of the MBW complex in tobacco flowers and affects germination through adjustment of PA concentrations and ABA/GA signaling in tobacco seeds.

摘要

原花青素（PAs）是重要的酚类化合物，PA生物合成受由R2R3-MYB调节因子、bHLH因子和WDR蛋白组成的三元MBW复合体调控。在本研究中，烟草R2R3-MYB因子NtMYB330被鉴定为PA特异性调节因子，在过表达株系的花中PA生物合成增强，而在突变体的花中PA生物合成减弱。NtMYB330可与类黄酮相关的bHLH伴侣NtAn1b和WDR蛋白NtAn11-1相互作用，且NtMYB330-NtAn1b复合体是实现PA相关结构基因、、和强转录激活所必需的。我们的数据表明，NtMYB330调节种子中的PA生物合成并影响种子萌发，其中过表达株系在种皮中PA积累较高且萌发受到抑制，而突变体种皮PA减少且萌发得到改善。NtMYB330可能通过两种机制影响种子萌发：调节种皮PA以影响种皮引起的休眠。此外，NtMYB330调节脱落酸（ABA）和赤霉素（GA）信号相关基因的表达，影响ABA-GA相互作用和种子萌发。本研究表明，NtMYB330通过在烟草花中形成MBW复合体特异性调节PA生物合成，并通过调节烟草种子中PA浓度和ABA/GA信号影响种子萌发。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e80/8801704/7a3827967113/fpls-12-819247-g009.jpg
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