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活性氧与赤霉素相互诱导调控烟草种子萌发

Reactive Oxygen Species and Gibberellin Acid Mutual Induction to Regulate Tobacco Seed Germination.

作者信息

Li Zhan, Gao Yue, Zhang Yuchan, Lin Cheng, Gong Dongting, Guan Yajing, Hu Jin

机构信息

Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.

出版信息

Front Plant Sci. 2018 Oct 2;9:1279. doi: 10.3389/fpls.2018.01279. eCollection 2018.

DOI:10.3389/fpls.2018.01279
PMID:30356911
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6190896/
Abstract

Seed germination is a complex process controlled by various mechanisms. To examine the potential contribution of reactive oxygen species (ROS) and gibberellin acid (GA) in regulating seed germination, diphenylene iodonium chloride (DPI) and uniconazole (Uni), as hydrogen peroxide (HO) and GA synthesis inhibitor, respectively, were exogenously applied on tobacco seeds using the seed priming method. Seed priming with DPI or Uni decreased germination percentage as compared with priming with HO, especially the DPI + Uni combination. HO and GA completely reversed the inhibition caused by DPI or Uni. The germination percentages with HO + Uni and GA + DPI combinations kept the same level as with HO. Meanwhile, GA or HO increased GA content and deceased ABA content through corresponding gene expressions involving homeostasis and signal transduction. In addition, the activation of storage reserve mobilization and the enhancement of soluble sugar content and isocitrate lyase (ICL) activity were also induced by GA or HO. These results strongly suggested that HO and GA were essential for tobacco seed germination and by downregulating the ABA/GA ratio and inducing reserve composition mobilization mutually promoted seed germination. Meanwhile, ICL activity was jointly enhanced by a lower ABA/GA ratio and a higher ROS concentration.

摘要

种子萌发是一个受多种机制控制的复杂过程。为了研究活性氧（ROS）和赤霉素（GA）在调节种子萌发中的潜在作用，分别使用种子引发方法将作为过氧化氢（HO）和GA合成抑制剂的二苯基氯化碘鎓（DPI）和烯效唑（Uni）外源施加到烟草种子上。与用HO引发相比，用DPI或Uni引发降低了发芽率，尤其是DPI + Uni组合。HO和GA完全逆转了由DPI或Uni引起的抑制作用。HO + Uni和GA + DPI组合的发芽率与用HO处理时保持相同水平。同时，GA或HO通过涉及稳态和信号转导的相应基因表达增加了GA含量并降低了ABA含量。此外，GA或HO还诱导了贮藏物质动员的激活以及可溶性糖含量和异柠檬酸裂解酶（ICL）活性的增强。这些结果强烈表明，HO和GA对烟草种子萌发至关重要，并且通过下调ABA/GA比值和诱导贮藏物质组成动员来相互促进种子萌发。同时，较低的ABA/GA比值和较高的ROS浓度共同增强了ICL活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5047/6190896/b9ea8c4f9579/fpls-09-01279-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5047/6190896/ddffc3734e9f/fpls-09-01279-g006.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5047/6190896/c5ab09b6cb6e/fpls-09-01279-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5047/6190896/843df71e9cc3/fpls-09-01279-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5047/6190896/87d31b6e09d1/fpls-09-01279-g003.jpg
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