Chmielewska-Krzesińska Małgorzata, Wąsowicz Krzysztof
Department of Pathophysiology, Forensic Veterinary Medicine and Administration, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
J Vet Res. 2021 Oct 6;65(4):513-517. doi: 10.2478/jvetres-2021-0051. eCollection 2021 Dec.
Ozone is not harmful itself; however, it directly oxidises biomolecules and produces radical-dependent cytotoxicity. Exposure to ozone is by inhalation and therefore the lungs develop the main anti-inflammatory response, while ozone has an indirect impact on the other organs. This study investigated the local and systemic effects of the ozone-associated inflammatory response.
Three groups each of 5 Wistar Han rats aged 6 months were exposed for 2h to airborne ozone at 0.5 ppm and a fourth identical group were unexposed controls. Sacrifice was at 3h after exposure for control rats and one experimental group and at 24 h and 48 h for the others. Lung and liver samples were evaluated for changes in expression of transforming growth factor beta 1, anti-inflammatory interleukin 10, pro-inflammatory tumour necrosis factor alpha and interleukin 1 beta and two nuclear factor kappa-light-chain-enhancer of B cells subunit genes. Total RNA was isolated from the samples in spin columns and cDNA was synthesised in an RT-PCR. Expression levels were compared to those of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and analysed statistically.
All variables changed non-linearly over time comparing experimental groups to the control. Conspicuous expression changes in the subunit genes and cytokines were observed in both evaluated organs.
Locally and systemically, inflammation responses to ozone inhalation include regulation of certain genes' expression. The mechanisms are unalike in lungs and liver but ozone exerts a similar effect in both organs. A broader range of variables influential on ozone response should be studied in the future.
臭氧本身并无危害；然而，它会直接氧化生物分子并产生自由基依赖性细胞毒性。臭氧通过吸入进入人体，因此肺部会产生主要的抗炎反应，而臭氧对其他器官有间接影响。本研究调查了与臭氧相关的炎症反应的局部和全身影响。
将三组，每组5只6个月大的Wistar Han大鼠暴露于0.5 ppm的空气中臭氧中2小时，第四组相同的大鼠作为未暴露的对照组。对照组大鼠和一个实验组在暴露后3小时处死，其他组在24小时和48小时处死。对肺和肝样本进行评估，以检测转化生长因子β1、抗炎性白细胞介素10、促炎性肿瘤坏死因子α和白细胞介素1β的表达变化以及两个B细胞亚基基因的核因子κB轻链增强子。从样本中在旋转柱中分离总RNA，并在逆转录聚合酶链反应（RT-PCR）中合成cDNA。将表达水平与甘油醛-3-磷酸脱氢酶（GAPDH）的表达水平进行比较，并进行统计学分析。
与对照组相比，所有变量随时间呈非线性变化。在两个评估器官中均观察到亚基基因和细胞因子的明显表达变化。
局部和全身而言，吸入臭氧后的炎症反应包括某些基因表达的调节。肺和肝中的机制不同，但臭氧在两个器官中都发挥了类似的作用。未来应研究更广泛的影响臭氧反应的变量。
