Department of Neurobiology and Anatomy, Wake Forest School of Medicine, Winston-Salem, NC 27157, USA.
Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA.
STAR Protoc. 2022 Jan 22;3(1):101136. doi: 10.1016/j.xpro.2022.101136. eCollection 2022 Mar 18.
We present an experimental protocol to record neuronal activity during intermittent stimulation of nucleus basalis (NB), as macaque monkeys perform cognitive tasks. This protocol includes implantation of electrodes and generator devices to deliver electrical stimulation to NB using multiple approaches in monkeys. Direct stimulation of NB avoids peripheral cholinergic side effects, optimizes timing, and activates non-cholinergic projection neurons. We describe electrode preparation, surgery, and implantation for direct evaluation of how stimulation affects monkeys' behavior and neuronal activity. For complete details on the use and execution of this profile, please refer to Qi et al. (2021).
我们提出了一个实验方案，记录猕猴在基底核（NB）间歇性刺激期间的神经元活动，猕猴在执行认知任务时。该方案包括在猴子中使用多种方法植入电极和发生器装置，以向 NB 提供电刺激。NB 的直接刺激可避免外周胆碱能副作用，优化时间，并激活非胆碱能投射神经元。我们描述了电极的准备、手术和植入，以直接评估刺激如何影响猴子的行为和神经元活动。有关此配置文件的使用和执行的完整详细信息，请参阅 Qi 等人。（2021 年）。
