酿酒酵母谷胱甘肽缺陷型突变体的分离。
Isolation of glutathione-deficient mutants of the yeast Saccharomyces cerevisiae.
作者信息
Kistler M, Summer K H, Eckardt F
出版信息
Mutat Res. 1986 Feb;173(2):117-20. doi: 10.1016/0165-7992(86)90087-4.
Glutathione-deficient (gsh-) mutants of the yeast Saccharomyces cerevisiae were isolated after UV treatment using MNNG as selective agent. For genetic and biochemical characterization 5 mutant strains were chosen which exhibited considerably decreased residual GSH contents varying from 2 to 6% of the wild-type levels. All 5 isolates showed a 2:2 segregation of the gsh-:GSH+ phenotypes alluding to a monogenic recessive mutation. Complementation analysis indicates that all gsh- mutants belong to one complementation group.
使用N-甲基-N'-硝基-N-亚硝基胍（MNNG）作为选择剂，经紫外线处理后分离出酿酒酵母的谷胱甘肽缺陷型（gsh-）突变体。为了进行遗传和生化特性分析，选择了5个突变菌株，它们的谷胱甘肽（GSH）残留量大幅降低，仅为野生型水平的2%至6%。所有5个分离株的gsh-:GSH+表型均呈现2:2分离，这暗示着单基因隐性突变。互补分析表明，所有gsh-突变体都属于一个互补群。
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