• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

甘草中的天然三萜类化合物能有效抑制 SARS-CoV-2 感染。

Natural triterpenoids from licorice potently inhibit SARS-CoV-2 infection.

机构信息

State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China.

CAS Key Laboratory of Special Pathogens and Biosafety, Centre for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.

出版信息

J Adv Res. 2021 Nov 26;36:201-210. doi: 10.1016/j.jare.2021.11.012. eCollection 2022 Feb.

DOI:10.1016/j.jare.2021.11.012
PMID:35116174
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8620242/
Abstract

INTRODUCTION

The COVID-19 global epidemic caused by severe acute respiratory syndrome coronavirus (SARS-CoV-2) is a great public health emergency. Discovering antiviral drug candidates is urgent for the prevention and treatment of COVID-19.

OBJECTIVES

This work aims to discover natural SARS-CoV-2 inhibitors from the traditional Chinese herbal medicine licorice.

METHODS

We screened 125 small molecules from Fisch. (licorice, Gan-Cao) by virtual ligand screening targeting the receptor-binding domain (RBD) of SARS-CoV-2 spike protein. Potential hit compounds were further evaluated by ELISA, SPR, luciferase assay, antiviral assay and pharmacokinetic study.

RESULTS

The triterpenoids licorice-saponin A3 (A3) and glycyrrhetinic acid (GA) could potently inhibit SARS-CoV-2 infection, with EC of 75 nM and 3.17 µM, respectively. Moreover, we reveal that A3 mainly targets the nsp7 protein, and GA binds to the spike protein RBD of SARS-CoV-2.

CONCLUSION

In this work, we found GA and A3 from licorice potently inhibit SARS-CoV-2 infection by affecting entry and replication of the virus. Our findings indicate that these triterpenoids may contribute to the clinical efficacy of licorice for COVID-19 and could be promising candidates for antiviral drug development.

摘要

简介

由严重急性呼吸系统综合征冠状病毒 2 型（SARS-CoV-2）引起的 COVID-19 全球疫情是一场重大的公共卫生紧急事件。发现抗病毒药物候选物对于 COVID-19 的预防和治疗至关重要。

目的

本研究旨在从传统中药甘草中发现天然 SARS-CoV-2 抑制剂。

方法

我们通过针对 SARS-CoV-2 刺突蛋白受体结合域（RBD）的虚拟配体筛选，从 Fisch.（甘草，甘草）中筛选了 125 种小分子。通过 ELISA、SPR、荧光素酶测定、抗病毒测定和药代动力学研究进一步评估潜在的命中化合物。

结果

三萜甘草皂苷 A3（A3）和甘草酸（GA）能够强烈抑制 SARS-CoV-2 感染，EC50 分别为 75 nM 和 3.17 µM。此外，我们发现 A3 主要靶向 nsp7 蛋白，而 GA 与 SARS-CoV-2 的刺突蛋白 RBD 结合。

结论

在这项工作中，我们发现甘草中的 GA 和 A3 通过影响病毒的进入和复制，强烈抑制 SARS-CoV-2 感染。我们的研究结果表明，这些三萜类化合物可能有助于甘草治疗 COVID-19 的临床疗效，并且可能是有前途的抗病毒药物开发候选物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e0/8800042/77ea4e71ed8a/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e0/8800042/6e359f4b4ba3/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e0/8800042/1d12b70bc1ac/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e0/8800042/319403667b63/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e0/8800042/aae8092ff01e/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e0/8800042/4d274f651d1e/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e0/8800042/77ea4e71ed8a/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e0/8800042/6e359f4b4ba3/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e0/8800042/1d12b70bc1ac/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e0/8800042/319403667b63/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e0/8800042/aae8092ff01e/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e0/8800042/4d274f651d1e/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e0/8800042/77ea4e71ed8a/gr5.jpg

相似文献

1
Natural triterpenoids from licorice potently inhibit SARS-CoV-2 infection.甘草中的天然三萜类化合物能有效抑制 SARS-CoV-2 感染。
J Adv Res. 2021 Nov 26;36:201-210. doi: 10.1016/j.jare.2021.11.012. eCollection 2022 Feb.
2
Licorice-saponin A3 is a broad-spectrum inhibitor for COVID-19 by targeting viral spike and anti-inflammation.甘草皂苷A3是一种通过靶向病毒刺突蛋白和抗炎作用来抑制新型冠状病毒肺炎的广谱抑制剂。
J Pharm Anal. 2023 May 22;14(1):115-27. doi: 10.1016/j.jpha.2023.05.011.
3
Can Antiviral Activity of Licorice Help Fight COVID-19 Infection?甘草的抗病毒活性能帮助对抗 COVID-19 感染吗？
Biomolecules. 2021 Jun 8;11(6):855. doi: 10.3390/biom11060855.
4
Multidisciplinary Approaches Identify Compounds that Bind to Human ACE2 or SARS-CoV-2 Spike Protein as Candidates to Block SARS-CoV-2-ACE2 Receptor Interactions.多学科方法鉴定与人 ACE2 或 SARS-CoV-2 刺突蛋白结合的化合物，作为阻断 SARS-CoV-2-ACE2 受体相互作用的候选药物。
mBio. 2021 Mar 30;12(2):e03681-20. doi: 10.1128/mBio.03681-20.
5
In silico ADMET and molecular docking study on searching potential inhibitors from limonoids and triterpenoids for COVID-19.计算机辅助的 ADMET 和分子对接研究，寻找来自柠檬苦素和三萜类化合物的 COVID-19 潜在抑制剂。
Comput Biol Med. 2020 Sep;124:103936. doi: 10.1016/j.compbiomed.2020.103936. Epub 2020 Jul 28.
6
Glycyrrhizic Acid Inhibits SARS-CoV-2 Infection by Blocking Spike Protein-Mediated Cell Attachment.甘草酸通过阻断刺突蛋白介导的细胞附着来抑制新型冠状病毒2感染。
Molecules. 2021 Oct 9;26(20):6090. doi: 10.3390/molecules26206090.
7
[Anti-virus research of triterpenoids in licorice].[甘草中三萜类化合物的抗病毒研究]
Bing Du Xue Bao. 2013 Nov;29(6):673-9.
8
Exploring Spike Protein as Potential Target of Novel Coronavirus and to Inhibit the Viability Utilizing Natural Agents.探索刺突蛋白作为新型冠状病毒的潜在靶点，并利用天然药物抑制其活性。
Curr Drug Targets. 2021;22(17):2006-2020. doi: 10.2174/1389450122666210309105820.
9
L. Extracts and Other Therapeutics against SARS-CoV-2 in Central Eurasia: Available but Overlooked.中亚地区针对 SARS-CoV-2 的提取物和其他疗法：已有但被忽视。
Molecules. 2023 Aug 19;28(16):6142. doi: 10.3390/molecules28166142.
10
The spike-ACE2 binding assay: An in vitro platform for evaluating vaccination efficacy and for screening SARS-CoV-2 inhibitors and neutralizing antibodies.刺突蛋白-ACE2 结合分析：评估疫苗效力和筛选 SARS-CoV-2 抑制剂及中和抗体的体外平台。
J Immunol Methods. 2022 Apr;503:113244. doi: 10.1016/j.jim.2022.113244. Epub 2022 Feb 23.

引用本文的文献

1
Selected Pentacyclic Triterpenoids and Their Derivatives as Biologically Active Compounds.作为生物活性化合物的选定五环三萜类化合物及其衍生物
Molecules. 2025 Jul 24;30(15):3106. doi: 10.3390/molecules30153106.
2
An advanced network pharmacology investigation of Shufeng Jiedu formula in the treatment for respiratory tract infection based on LC-MS, data mining, molecular docking, and molecular dynamic simulation.基于液相色谱-质谱联用、数据挖掘、分子对接和分子动力学模拟的疏风解毒方治疗呼吸道感染的网络药理学深入研究
Brief Bioinform. 2025 Jul 2;26(4). doi: 10.1093/bib/bbaf353.
3
Screening criteria for high-quality Glycyrrhiza uralensis Fisch. seeds from the Ordos Plateau.

本文引用的文献

1
Shedding of infectious SARS-CoV-2 despite vaccination.接种疫苗后仍有传染性 SARS-CoV-2 的脱落。
PLoS Pathog. 2022 Sep 30;18(9):e1010876. doi: 10.1371/journal.ppat.1010876. eCollection 2022 Sep.
2
The SARS-CoV-2 Lambda variant exhibits enhanced infectivity and immune resistance.SARS-CoV-2 Lambda 变体表现出增强的感染力和免疫抗性。
Cell Rep. 2022 Jan 11;38(2):110218. doi: 10.1016/j.celrep.2021.110218. Epub 2021 Dec 18.
3
Mechanisms of SARS-CoV-2 entry into cells.SARS-CoV-2 进入细胞的机制。
鄂尔多斯高原优质乌拉尔甘草种子的筛选标准。
Sci Rep. 2025 Jul 1;15(1):21955. doi: 10.1038/s41598-025-05736-0.
4
Exploring anti-SARS-CoV-2 natural products: dual-viral target inhibition by delphinidin and the anti-coronaviral efficacy of deapio platycodin D.探索抗SARS-CoV-2天然产物：飞燕草素的双病毒靶点抑制作用及去甲桔梗皂苷D的抗冠状病毒功效
Nat Prod Bioprospect. 2025 Jun 13;15(1):39. doi: 10.1007/s13659-025-00523-w.
5
Progress of cGAS-STING signaling pathway-based modulation of immune response by traditional Chinese medicine in clinical diseases.基于cGAS-STING信号通路的中药对临床疾病免疫反应调节的研究进展
Front Immunol. 2024 Dec 16;15:1510628. doi: 10.3389/fimmu.2024.1510628. eCollection 2024.
6
Jinhua Qinggan Granule UHPLC-Q-extractive-Orbitrap-MS assay: Putative identification of 45 potential anti-Covid-19 constituents, confidential addition, and pharmacopoeia quality-markers recommendation.金花清感颗粒超高效液相色谱-四级杆-静电场轨道阱高分辨质谱分析：45种潜在抗新冠病毒成分的推定鉴定、补充机密信息及药典质量标志物推荐
J Food Drug Anal. 2023 Aug 31;31(3):534-551. doi: 10.38212/2224-6614.3466.
7
Amides of moronic acid and morolic acid with the tripeptides MAG and GAM targeting antimicrobial, antiviral and cytotoxic effects.丙二酸和莫罗酸与三肽MAG和GAM形成的酰胺，具有抗菌、抗病毒和细胞毒性作用。
RSC Med Chem. 2024 Oct 29;16(2):801-811. doi: 10.1039/d4md00742e. eCollection 2025 Feb 19.
8
Effect of SiHuangQingXinWan on -induced pneumonia: mechanistic insights.四黄清心丸对[具体因素]诱导的肺炎的影响：机制洞察
Front Pharmacol. 2024 Oct 15;15:1444439. doi: 10.3389/fphar.2024.1444439. eCollection 2024.
9
Research progress of traditional Chinese medicine compound "Chaihu Shugan Powder" in the treatment of premenstrual syndrome.中药复方“柴胡疏肝散”治疗经前期综合征的研究进展。
Medicine (Baltimore). 2024 Oct 4;103(40):e38351. doi: 10.1097/MD.0000000000038351.
10
Natural 7,8-secolignans from fruit potently inhibit SARS-CoV-2 3CL and inflammation.来自水果的天然7,8-裂环木脂素可有效抑制SARS-CoV-2 3CL蛋白酶和炎症。
J Tradit Complement Med. 2024 Jan 16;14(5):501-509. doi: 10.1016/j.jtcme.2024.01.005. eCollection 2024 Sep.
Nat Rev Mol Cell Biol. 2022 Jan;23(1):3-20. doi: 10.1038/s41580-021-00418-x. Epub 2021 Oct 5.
4
An overview of potential inhibitors targeting non-structural proteins 3 (PL and Mac1) and 5 (3CL/M) of SARS-CoV-2.靶向严重急性呼吸综合征冠状病毒2（SARS-CoV-2）非结构蛋白3（PL和Mac1）和5（3CL/M）的潜在抑制剂概述。
Comput Struct Biotechnol J. 2021;19:4868-4883. doi: 10.1016/j.csbj.2021.08.036. Epub 2021 Aug 24.
5
X-ray screening identifies active site and allosteric inhibitors of SARS-CoV-2 main protease.X射线筛选鉴定出新型冠状病毒主要蛋白酶的活性位点和变构抑制剂。
Science. 2021 May 7;372(6542):642-646. doi: 10.1126/science.abf7945. Epub 2021 Apr 2.
6
SARS-CoV-2 M inhibitors with antiviral activity in a transgenic mouse model.具有抗病毒活性的 SARS-CoV-2 M 抑制剂在转基因小鼠模型中。
Science. 2021 Mar 26;371(6536):1374-1378. doi: 10.1126/science.abf1611. Epub 2021 Feb 18.
7
Repurposed Antiviral Drugs for Covid-19 - Interim WHO Solidarity Trial Results.用于治疗新冠肺炎的抗病毒药物 repurposed - 世界卫生组织团结试验中期结果
N Engl J Med. 2021 Feb 11;384(6):497-511. doi: 10.1056/NEJMoa2023184. Epub 2020 Dec 2.
8
Dalbavancin binds ACE2 to block its interaction with SARS-CoV-2 spike protein and is effective in inhibiting SARS-CoV-2 infection in animal models.达巴万星通过与 ACE2 结合来阻断其与 SARS-CoV-2 刺突蛋白的相互作用，并且在动物模型中对抑制 SARS-CoV-2 感染有效。
Cell Res. 2021 Jan;31(1):17-24. doi: 10.1038/s41422-020-00450-0. Epub 2020 Dec 1.
9
The place for remdesivir in COVID-19 treatment.瑞德西韦在新冠病毒肺炎治疗中的地位。
Lancet Infect Dis. 2021 Jan;21(1):20-21. doi: 10.1016/S1473-3099(20)30911-7. Epub 2020 Nov 26.
10
The effect of whey protein on viral infection and replication of SARS-CoV-2 and pangolin coronavirus in vitro.乳清蛋白对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）和穿山甲冠状病毒体外感染及复制的影响。
Signal Transduct Target Ther. 2020 Nov 24;5(1):275. doi: 10.1038/s41392-020-00408-z.