Zhao Jing, Zhang Pu, Wang Xin
Department of Otolaryngology, The Third Hospital of Hebei Medical University, Shijiazhuang, China.
Transl Cancer Res. 2021 Nov;10(11):4859-4869. doi: 10.21037/tcr-21-2087.
Laryngeal squamous cell carcinoma (LSCC) is one of the most commonly seen malignancies of the head and neck, with increasing incidence and mortality. The Y-box-binding protein 1 (YBX1) is a type of oncoprotein which is related to the malignant phenotype of many cancers. It is reported that YBX1 could regulate tumorigenesis, recurrence, and metastasis in multiple cancers. However, little is known about its carcinogenic function and mechanism in LSCC.
Firstly, Through Oncomine StarBase, we found that the YBX1 mRNA level was increased in a variety of cancer tissues, including in the LSCC, compared with normal tissues. We silenced YBX1 in LSCC cells using short hairpin RNAs (shRNAs). Secondly, the biological function of YBX1 in LSCC cells was examined by the Cell Counting Kit-8 (CCK-8) assay, flow cytometry, the wound healing assay, and the transwell assay. Thirdly, the correlation between YBX1 and the PI3K/AKT pathway was verified by the western blot assay.
Expression of YBX1 is higher in a variety of cancer tissues, especially in the head and neck cancers. After transfected with lentiviral vectors, the expression of YBX1 was significantly silenced. Functionally, low expression of YBX1 promoted LSCC cell apoptosis and inhibited LSCC cell proliferation, migration, and invasion. The transfection of sh-YBX1 resulted in an obvious decrease in PI3K/AKT signaling molecules in LSCC cells.
We demonstrated that YBX1 could promote LSCC cell progression through the PI3K/AKT pathway, providing new insights into a potential biomarker and target for LSCC treatment.
喉鳞状细胞癌（LSCC）是头颈部最常见的恶性肿瘤之一，其发病率和死亡率呈上升趋势。Y盒结合蛋白1（YBX1）是一种癌蛋白，与多种癌症的恶性表型相关。据报道，YBX1可调节多种癌症的肿瘤发生、复发和转移。然而，其在LSCC中的致癌功能和机制尚不清楚。
首先，通过Oncomine StarBase，我们发现与正常组织相比，YBX1 mRNA水平在包括LSCC在内的多种癌组织中升高。我们使用短发夹RNA（shRNA）在LSCC细胞中沉默YBX1。其次，通过细胞计数试剂盒-8（CCK-8）检测、流式细胞术、伤口愈合检测和transwell检测来研究YBX1在LSCC细胞中的生物学功能。第三，通过蛋白质印迹法验证YBX1与PI3K/AKT信号通路之间的相关性。
YBX1在多种癌组织中表达较高，尤其是在头颈部癌症中。转染慢病毒载体后，YBX1的表达明显沉默。在功能上，YBX1的低表达促进LSCC细胞凋亡并抑制LSCC细胞增殖、迁移和侵袭。sh-YBX1转染导致LSCC细胞中PI3K/AKT信号分子明显减少。
我们证明YBX1可通过PI3K/AKT信号通路促进LSCC细胞进展，为LSCC治疗的潜在生物标志物和靶点提供了新的见解。
