• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

门静脉注射冬凌草甲素对BALb/c裸鼠结肠癌肝转移半脾模型的预防和治疗作用

Preventive and therapeutic effect of intraportal oridonin on BALb/c nude mice hemispleen model of colon cancer liver metastasis.

作者信息

Yang Yu-Shen, Wen Dan, Zhao Xue-Feng

机构信息

Department of General Surgery, Dalian University Affiliated Xinhua Hospital, Dalian, China.

出版信息

Transl Cancer Res. 2021 Mar;10(3):1324-1335. doi: 10.21037/tcr-20-3042.

DOI:10.21037/tcr-20-3042
PMID:35116458
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8798652/
Abstract

BACKGROUND

This study is to investigate the preventive and therapeutic effect of intraportal oridonin on colorectal cancer liver metastasis (CRCLM).

METHODS

The inhibitory effect of oridonin on HT29 cells was determined by CCK-8 and MTT assays. The preventive and therapeutic effect of intraportal oridonin on CRCLM were investigated by establishing BALb/c nude mice hemispleen models of colon cancer liver metastasis. The microscopic characteristics of tumor tissues were observed by hematoxylin-eosin staining, immunohistochemistry and TUNEL staining. On the other hand, liver function enzymes, such as alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP), were detected to evaluate the hepatotoxicity of intraportal oridonin. The serum levels of tumor markers, including carcinoembryonic antigen (CEA) and α-fetoprotein (AFP), were used to investigate the intervention effect of intraportal oridonin on CRCLM.

RESULTS

Oridonin exerted an inhibitory effect on the proliferation of HT29 cells . Intraportal oridonin was found to effectively prevent the occurrence and formation of CRCLM, whilst intraportal oridonin can also exert a therapeutic effect on CRCLM. Additionally, liver enzymes testing indicated that intraportal oridonin possesses non-hepatotoxicity, instead can effectively alleviate liver injury caused by tumor. Furthermore, intraportal oridonin was also revealed to decrease the serum levels of AFP and CEA.

CONCLUSIONS

Intraportal oridonin can effectively inhibit the formation of liver metastatic tumor and exert a certain degree of preventive and therapeutic effect on CRCLM. These findings indicate intraportal oridonin to be a promising anti-metastasis agent for CRCLM.

摘要

背景

本研究旨在探讨门静脉注射冬凌草甲素对结直肠癌肝转移（CRCLM）的预防和治疗作用。

方法

采用CCK-8和MTT法检测冬凌草甲素对HT29细胞的抑制作用。通过建立BALb/c裸鼠结肠癌肝转移半脾模型，研究门静脉注射冬凌草甲素对CRCLM的预防和治疗作用。采用苏木精-伊红染色、免疫组织化学和TUNEL染色观察肿瘤组织的微观特征。另一方面，检测肝功能酶，如丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）和碱性磷酸酶（ALP），以评估门静脉注射冬凌草甲素的肝毒性。采用癌胚抗原（CEA）和甲胎蛋白（AFP）等肿瘤标志物的血清水平，研究门静脉注射冬凌草甲素对CRCLM的干预作用。

结果

冬凌草甲素对HT29细胞的增殖具有抑制作用。发现门静脉注射冬凌草甲素能有效预防CRCLM的发生和形成，同时门静脉注射冬凌草甲素对CRCLM也有治疗作用。此外，肝酶检测表明门静脉注射冬凌草甲素无肝毒性，反而能有效减轻肿瘤引起的肝损伤。此外，还发现门静脉注射冬凌草甲素可降低AFP和CEA的血清水平。

结论

门静脉注射冬凌草甲素能有效抑制肝转移瘤的形成，对CRCLM有一定的预防和治疗作用。这些发现表明门静脉注射冬凌草甲素是一种有前途的CRCLM抗转移药物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7af9/8798652/07dfdc0d500a/tcr-10-03-1324-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7af9/8798652/65fe43b4aa5f/tcr-10-03-1324-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7af9/8798652/bc88e4623d1e/tcr-10-03-1324-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7af9/8798652/2dd66b225b55/tcr-10-03-1324-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7af9/8798652/62cacc2e9244/tcr-10-03-1324-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7af9/8798652/07dfdc0d500a/tcr-10-03-1324-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7af9/8798652/65fe43b4aa5f/tcr-10-03-1324-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7af9/8798652/bc88e4623d1e/tcr-10-03-1324-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7af9/8798652/2dd66b225b55/tcr-10-03-1324-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7af9/8798652/62cacc2e9244/tcr-10-03-1324-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7af9/8798652/07dfdc0d500a/tcr-10-03-1324-f5.jpg

相似文献

1
Preventive and therapeutic effect of intraportal oridonin on BALb/c nude mice hemispleen model of colon cancer liver metastasis.门静脉注射冬凌草甲素对BALb/c裸鼠结肠癌肝转移半脾模型的预防和治疗作用
Transl Cancer Res. 2021 Mar;10(3):1324-1335. doi: 10.21037/tcr-20-3042.
2
Oridonin Enhances the Anti-Metastasis Effect of Oxaliplatinliplatin on Colorectal Cancer Liver Metastasis.冬凌草甲素增强奥沙利铂对结直肠癌肝转移的抗转移作用。
Bull Exp Biol Med. 2021 Nov;172(1):26-32. doi: 10.1007/s10517-021-05324-y. Epub 2021 Nov 18.
3
Sophocarpine can enhance the inhibiting effect of oxaliplatin on colon cancer liver metastasis-in vitro and in vivo. sophocarpine 能增强奥沙利铂对结直肠癌肝转移的抑制作用：体内外研究。
Naunyn Schmiedebergs Arch Pharmacol. 2021 Jun;394(6):1263-1274. doi: 10.1007/s00210-020-02032-8. Epub 2021 Jan 6.
4
Effects of oridonin on proliferation of HT29 human colon carcinoma cell lines both in vitro and in vivo in mice.冬凌草甲素对HT29人结肠癌细胞系在体外及小鼠体内增殖的影响。
Pharmazie. 2007 Jun;62(6):439-44.
5
The inhibitory effect of oridonin on colon cancer was mediated by deactivation of TGF-β1/Smads-PAI-1 signaling pathway in vitro and vivo.冬凌草甲素对结肠癌的抑制作用是通过在体内外使TGF-β1/Smads-PAI-1信号通路失活来介导的。
Onco Targets Ther. 2019 Sep 11;12:7467-7476. doi: 10.2147/OTT.S220401. eCollection 2019.
6
Oridonin induces apoptosis and senescence in colorectal cancer cells by increasing histone hyperacetylation and regulation of p16, p21, p27 and c-myc.冬凌草甲素通过增加组蛋白乙酰化和调控 p16、p21、p27 和 c-myc 诱导结直肠癌细胞凋亡和衰老。
BMC Cancer. 2010 Nov 6;10:610. doi: 10.1186/1471-2407-10-610.
7
Anti-tumor activity of oridonin on SNU-5 subcutaneous xenograft model via regulation of c-Met pathway.冬凌草甲素通过调控c-Met通路对SNU-5皮下异种移植模型的抗肿瘤活性
Tumour Biol. 2014 Sep;35(9):9139-46. doi: 10.1007/s13277-014-2178-4. Epub 2014 Jun 12.
8
Oridonin inhibits the migration and epithelial-to-mesenchymal transition of small cell lung cancer cells by suppressing FAK-ERK1/2 signalling pathway.冬凌草甲素通过抑制 FAK-ERK1/2 信号通路抑制小细胞肺癌细胞的迁移和上皮-间质转化。
J Cell Mol Med. 2020 Apr;24(8):4480-4493. doi: 10.1111/jcmm.15106. Epub 2020 Mar 13.
9
Oridonin triggers apoptosis in colorectal carcinoma cells and suppression of microRNA-32 expression augments oridonin-mediated apoptotic effects.冬凌草甲素可诱导结肠癌细胞凋亡，而抑制微小RNA-32的表达可增强冬凌草甲素介导的凋亡作用。
Biomed Pharmacother. 2015 May;72:125-34. doi: 10.1016/j.biopha.2015.04.016. Epub 2015 Apr 27.
10
Oridonin induces apoptosis and cell cycle arrest of gallbladder cancer cells via the mitochondrial pathway.冬凌草甲素通过线粒体途径诱导胆囊癌细胞凋亡和细胞周期停滞。
BMC Cancer. 2014 Mar 21;14:217. doi: 10.1186/1471-2407-14-217.

引用本文的文献

1
Isodon rubescens research literature based on Web of Science database for visual analysis: A review.基于Web of Science数据库的冬凌草研究文献可视化分析：综述
Medicine (Baltimore). 2025 May 2;104(18):e41945. doi: 10.1097/MD.0000000000041945.
2
Evaluation of Serum Fructosamine as Diagnostic Marker of Postoperative Recurrence in the Patients with Breast Cancer.评估血清果糖胺作为乳腺癌患者术后复发的诊断标志物。
Biomed Res Int. 2023 Jul 12;2023:6435776. doi: 10.1155/2023/6435776. eCollection 2023.
3
Extracellular Vesicles and the Inflammasome: An Intricate Network Sustaining Chemoresistance.

本文引用的文献

1
Hepatoprotective effects of ethanolic extract of Boerhaavia diffusa against oxaliplatin induced hepatotoxicity in albino rats.白花蛇舌草乙醇提取物对奥沙利铂诱导的白化病大鼠肝毒性的保肝作用。
Pak J Pharm Sci. 2019 Sep;32(5):1927-1932.
2
A thiazole-derived oridonin analogue exhibits antitumor activity by directly and allosterically inhibiting STAT3.一种噻唑衍生的冬凌草甲素类似物通过直接和变构抑制 STAT3 发挥抗肿瘤活性。
J Biol Chem. 2019 Nov 15;294(46):17471-17486. doi: 10.1074/jbc.RA119.009801. Epub 2019 Oct 8.
3
The primary tumor location impacts survival outcome of colorectal liver metastases after hepatic resection: A systematic review and meta-analysis.
细胞外囊泡与炎性小体：维持化疗耐药性的复杂网络
Front Oncol. 2022 Apr 22;12:888135. doi: 10.3389/fonc.2022.888135. eCollection 2022.
原发肿瘤位置对肝切除术后结直肠肝转移的生存结局有影响：一项系统评价和荟萃分析。
Eur J Surg Oncol. 2019 Aug;45(8):1349-1356. doi: 10.1016/j.ejso.2019.04.017. Epub 2019 Apr 25.
4
Enhancement of chemosensitivity in 5-fluorouracil-resistant colon cancer cells with carcinoembryonic antigen-specific RNA aptamer.用癌胚抗原特异性 RNA 适体增强氟尿嘧啶耐药结肠癌细胞的化疗敏感性。
Mol Biol Rep. 2019 Aug;46(4):3835-3842. doi: 10.1007/s11033-019-04826-3. Epub 2019 Apr 26.
5
Anticancer effects of oridonin on colon cancer are mediated via BMP7/p38 MAPK/p53 signaling.冬凌草甲素通过 BMP7/p38 MAPK/p53 信号通路介导对结肠癌的抗癌作用。
Int J Oncol. 2018 Nov;53(5):2091-2101. doi: 10.3892/ijo.2018.4527. Epub 2018 Aug 16.
6
Drug-Loaded PLGA Electrospraying Porous Microspheres for the Local Therapy of Primary Lung Cancer via Pulmonary Delivery.用于原发性肺癌肺部给药局部治疗的载药聚乳酸-羟基乙酸共聚物电喷雾多孔微球
ACS Omega. 2017 May 31;2(5):2273-2279. doi: 10.1021/acsomega.7b00456. Epub 2017 May 24.
7
Therapeutic Potential of Oridonin and Its Analogs: From Anticancer and Antiinflammation to Neuroprotection.冬凌草甲素及其类似物的治疗潜力：从抗癌和抗炎到神经保护。
Molecules. 2018 Feb 22;23(2):474. doi: 10.3390/molecules23020474.
8
Oridonin induces apoptosis and reverses drug resistance in cisplatin resistant human gastric cancer cells.冬凌草甲素诱导顺铂耐药的人胃癌细胞凋亡并逆转其耐药性。
Oncol Lett. 2017 Aug;14(2):2499-2504. doi: 10.3892/ol.2017.6421. Epub 2017 Jun 19.
9
Oridonin induces autophagy via inhibition of glucose metabolism in p53-mutated colorectal cancer cells.冬凌草甲素通过抑制 p53 突变型结直肠癌细胞的糖代谢诱导自噬。
Cell Death Dis. 2017 Feb 23;8(2):e2633. doi: 10.1038/cddis.2017.35.
10
Molecular Insight in the Multifunctional Effects of Oridonin.冬凌草甲素多功能作用的分子洞察
Drugs R D. 2015 Sep;15(3):233-44. doi: 10.1007/s40268-015-0102-z.