Infection of mosquitoes with Plasmodium vivax from chimpanzees using membrane feeding.

Six splenectomized chimpanzees were infected with the Chesson or the North Korean strains of Plasmodium vivax. Heparinized blood taken from the animals was fed to approximately 45,000 mosquitoes using parafilm membranes. High-level mosquito infections were obtained with the blood from 4 animals. One animal infected mosquitoes only at a very low level. The other chimpanzee failed to produce a parasitemia high enough to warrant mosquito feeding.