Ponnudurai T, Lensen A H, van Gemert G J, Bolmer M, van Belkum A, van Eerd P, Mons B
Department of Parasitology, University of Nijmegen, The Netherlands.
Parasitology. 1990 Dec;101 Pt 3:317-20. doi: 10.1017/s0031182000060492.
Mass-scale production of Plasmodium vivax sporozoites in Anopheles stephensi was achieved using the chimpanzee (Pan troglodytes) as a source of infective blood. Membrane feeding was as successful as feeding mosquitoes directly on the animal so long as the time between drawing the blood and feeding was restricted to 45 min. Longer delays such as 2-3 h resulted in loss of infectivity in terms of oocyst production. The selected strain of A. stephensi was highly susceptible to P. vivax (Chesson strain). A strain of A. stephensi relatively refractory to P. falciparum showed no cross-refractoriness to P. vivax. Mixed infections of P. falciparum and P. vivax did not interfere with each other in their development in A. stephensi. A second normal blood meal to mosquitoes infected with P. vivax increased the yield of salivary gland sporozoites.
以黑猩猩（Pan troglodytes）作为感染性血液来源，实现了间日疟原虫子孢子在斯氏按蚊体内的大规模生产。只要采血与喂蚊之间的时间限制在45分钟以内，膜饲法与让蚊子直接吸食动物血液的效果一样好。更长时间的延迟，如2至3小时，会导致卵囊产生方面的感染性丧失。所选的斯氏按蚊品系对间日疟原虫（切森品系）高度敏感。对恶性疟原虫相对耐药的一个斯氏按蚊品系对间日疟原虫没有交叉耐药性。恶性疟原虫和间日疟原虫的混合感染在斯氏按蚊体内的发育过程中互不干扰。给感染间日疟原虫的蚊子提供第二顿正常血餐可提高唾液腺子孢子的产量。
