Li Tingting, Tian Yong, Wang Yixuan, Cui Zhen, He Zelai, Wu Xiao, Zhang Yajun, Jiang Hao
Department of Radiation Oncology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, China.
Department of Pharmacy, The First Affiliated Hospital of Bengbu Medical Collage, Bengbu, China.
Front Oncol. 2022 Jan 18;11:724251. doi: 10.3389/fonc.2021.724251. eCollection 2021.
Nasopharyngeal carcinoma (NPC) is a cancer that occurs in the nasopharynx. Infinite proliferation and distant metastasis are the main characteristics of NPC cells, and the main reason for the current failure of malignant tumor treatment. In this study, by integrating the immunohistochemical, cell transfection, western blot and real-time reverse transcriptase polymerase chain reaction (RT-PCR) analysis, we observed that the expression of KISS1 and its receptor gene (KISS1R) negatively related with the proliferation of NPC cells. Overexpression of the KISS1 genes in cells reduced cell proliferation, slow down the cell cycle, and increased apoptosis. Additionally, overexpression of these genes significantly increased Liver Kinase B1 (LKB1), phosphorylation of LKB1 and AMPK, indicated by Western blotting. Together, all of these results suggested for the first time that KISS1 and KISS1R suppress the proliferation of NPC cells by activating the LKB1/AMPK pathway, thus revealing a viable indicator for diagnosis of NPC in clinical practice.
鼻咽癌（NPC）是一种发生于鼻咽部的癌症。无限增殖和远处转移是NPC细胞的主要特征，也是当前恶性肿瘤治疗失败的主要原因。在本研究中，通过整合免疫组化、细胞转染、蛋白质免疫印迹及实时逆转录聚合酶链反应（RT-PCR）分析，我们观察到KISS1及其受体基因（KISS1R）的表达与NPC细胞的增殖呈负相关。细胞中KISS1基因的过表达降低了细胞增殖，减缓了细胞周期，并增加了细胞凋亡。此外，蛋白质免疫印迹显示，这些基因的过表达显著增加了肝脏激酶B1（LKB1）、LKB1的磷酸化水平及AMPK。总之，所有这些结果首次表明，KISS1和KISS1R通过激活LKB1/AMPK途径抑制NPC细胞的增殖，从而揭示了一种在临床实践中诊断NPC的可行指标。
