Department of Periodontology and Implantology, VYWS Dental College, Amravati, Maharashtra, India -
Department of Periodontology and Implantology, Sharad Pawar Dental College and Hospital, Wardha, Maharashtra, India.
Minerva Dent Oral Sci. 2022 Dec;71(6):346-352. doi: 10.23736/S2724-6329.22.04425-4. Epub 2022 Feb 4.
This study was carried out to evaluate and compare the levels of IL-35 in gingival crevicular fluid (GCF) in periodontally healthy subjects, patients with gingivitis and chronic periodontitis and to assess IL-35 as a marker for identification of periodontal disease activity.
GCF samples were obtained from periodontally healthy subjects (N.=15), gingivitis patients (N.=15) and patients with chronic periodontitis (N.=15). Clinical measurements like probing pocket depth, clinical attachment loss, bleeding on probing, Papillary Bleeding Index, and Modified Plaque Index were recorded. Enzyme-linked immunosorbent assay was used for the determination of GCF IL-35 levels in samples.
The IL-35 levels were significantly higher in the healthy subjects as compared to gingivitis and chronic periodontitis group. There was variation in GCF IL-35 levels in healthy sites in each group and gingivitis sites in gingivitis and chronic periodontitis patients.
The levels of IL-35 were observed to decrease with increase in the inflammatory status, so it might play a role in suppressing gingival inflammation and maintaining periodontal health.
本研究旨在评估和比较牙周健康受试者、牙龈炎患者和慢性牙周炎患者龈沟液（GCF）中的 IL-35 水平，并评估 IL-35 作为识别牙周疾病活动的标志物的作用。
从牙周健康受试者（N=15）、牙龈炎患者（N=15）和慢性牙周炎患者（N=15）中获取 GCF 样本。记录临床测量值，如探诊袋深度、临床附着丧失、探诊出血、乳头出血指数和改良菌斑指数。采用酶联免疫吸附试验测定样本中 GCF IL-35 水平。
与牙龈炎和慢性牙周炎组相比，健康受试者的 IL-35 水平明显更高。在每个组的健康部位和牙龈炎和慢性牙周炎患者的牙龈炎部位中，GCF IL-35 水平存在差异。
随着炎症状态的增加，观察到 IL-35 的水平下降，因此它可能在抑制牙龈炎症和维持牙周健康方面发挥作用。
