Ege University, School of Dentistry, Department of Periodontology, Izmir, Turkey.
Dis Markers. 2011;31(6):343-52. doi: 10.3233/DMA-2011-0849.
The aim of the present study was to investigate gingival crevicular fluid (GCF) calprotectin, osteocalcin and cross-linked N-terminal telopeptide (NTx) levels in health along with different periodontal diseases.
Twenty chronic periodontitis (CP), 20 generalized aggressive periodontitis (G-AgP), 20 gingivitis and 20 healthy subjects were included. Probing depth, clinical attachment level, plaque index and papillary bleeding index was recorded. GCF calprotectin, osteocalcin and NTx levels were analyzed by enzyme-linked immunosorbent assay (ELISA).
CP, G-AgP and gingivitis groups had higher GCF calprotectin total amount compared to healthy subjects (p< 0.008). CP and G-AgP groups had similar, but higher levels compared to gingivitis groups (p< 0.008). CP and G-AgP groups had lower GCF osteocalcin total amount compared to gingivitis and healthy groups (p< 0.008). CP group had higher GCF NTx but lower osteocalcin total amount and osteocalcin/NTx ratio than the G-AgP group (p< 0.008).
Our results suggest that elevated GCF calprotectin levels play a role as a reliable inflammatory marker in the pathogenesis of periodontal disease. Fluctuating GCF levels of osteocalcin and NTx might point out to the abnormal bone turnover in periodontitis. Our data document for the first time the role of NTx in the pathogenesis of different periodontal diseases.
本研究旨在探讨健康人群及不同牙周疾病患者龈沟液（GCF）钙卫蛋白、骨钙素和交联 N 端肽（NTx）水平。
纳入 20 例慢性牙周炎（CP）患者、20 例广泛性侵袭性牙周炎（G-AgP）患者、20 例牙龈炎患者和 20 例健康对照者。记录探诊深度、临床附着丧失、菌斑指数和牙龈出血指数。采用酶联免疫吸附试验（ELISA）分析 GCF 钙卫蛋白总浓度、骨钙素和 NTx 水平。
CP、G-AgP 和牙龈炎组的 GCF 钙卫蛋白总量均高于健康对照组（p<0.008）。CP 和 G-AgP 组的 GCF 钙卫蛋白总量相似，但高于牙龈炎组（p<0.008）。CP 和 G-AgP 组的 GCF 骨钙素总量低于牙龈炎组和健康对照组（p<0.008）。CP 组的 GCF NTx 较高，但骨钙素总量和骨钙素/NTx 比值低于 G-AgP 组（p<0.008）。
我们的结果表明，升高的 GCF 钙卫蛋白水平在牙周病的发病机制中作为一种可靠的炎症标志物发挥作用。骨钙素和 NTx 的 GCF 水平波动可能表明牙周炎中存在异常的骨转换。我们的数据首次证明了 NTx 在不同牙周疾病发病机制中的作用。
