Department of Marine Chemistry, College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan, China.
National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan, China.
Antonie Van Leeuwenhoek. 2022 Apr;115(4):473-486. doi: 10.1007/s10482-022-01710-2. Epub 2022 Feb 4.
Two yellow-pigmented bacterial strains, LZ-14 and ABI-LZ29, were isolated from the cultivable phycosphere microbiota of the highly toxic marine dinoflagellate Alexandrium catenella LZT09 and demonstrated obvious microalgae growth-promoting potentials toward the algal host. To elucidate the taxonomic status of the two bioactive bacterial strains, they were subjected to a polyphasic taxonomic characterization. Both strains were found to be Gram-negative, aerobic, rod-shaped and motile; to contain Q-10 as the predominant ubiquinone; summed feature 8, C, C ω7c 11-methyl and summed feature 3 as the major fatty acids; and diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and two unidentified phospholipids as the predominant polar lipids. Based on the phylogenetic analysis, phylogenomic inferences and phenotypic characteristics, the strains could be clearly distinguished from phylogenetically closely related species and formed a distinct monophyletic lineage in the family Rhodobacteraceae. The size of the draft genome of strain LZ-14 is 4.615 Mb, with a DNA G + C content of 63.3 mol%. It contains ten predicted secondary metabolite biosynthetic gene clusters and core genes for bacterial exopolysaccharide biosynthesis. Therefore, strain LZ-14 (= CCTCC AB 2017230 = KCTC 62342 ) represents a novel species of a new genus, for which the name Alexandriicola marinus gen. nov., sp. nov., is proposed.
从剧毒海洋甲藻亚历山大藻 LZT09 可培养的藻际微生物群中分离出两株黄色着色的细菌菌株 LZ-14 和 ABI-LZ29，它们对藻宿主表现出明显的促进藻类生长的潜力。为了阐明这两株具有生物活性的细菌菌株的分类地位，对它们进行了多相分类学特征描述。这两种细菌均为革兰氏阴性、需氧、杆状和运动性；含有 Q-10 作为主要的泛醌；主要脂肪酸为 sum feature 8、C、C ω7c 11-methyl 和 sum feature 3；主要极性脂为双磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油和两种未鉴定的磷脂。基于系统发育分析、系统发育推断和表型特征，这些菌株可以与系统发育上密切相关的种明显区分开来，并在红杆菌科中形成一个独特的单系进化枝。菌株 LZ-14 的基因组草图大小为 4.615 Mb，DNA G+C 含量为 63.3 mol%。它包含十个预测的次生代谢物生物合成基因簇和细菌胞外多糖生物合成的核心基因。因此，菌株 LZ-14（=CCTCC AB 2017230=KCTC 62342）代表了一个新属的新种，提议建立一个新属，命名为海洋亚历山大菌属 Alexandriicola marinus 属。
