Paediatric Dentistry and Orthodontics, Faculty of Dentistry, The University of Hong Kong, Hong Kong, SAR, China.
Sci Rep. 2022 Feb 4;12(1):1971. doi: 10.1038/s41598-022-06020-1.
Various materials are used in bone tissue engineering (BTE). Graphene oxide (GO) is a good candidate for BTE due to its antibacterial activity and biocompatibility. In this study, an innovative biomaterial consists of GO, agarose and hydroxyapatite (HA) was synthesized using electrophoresis system. The characterization of the synthesized biomaterial showed that needle-like crystals with high purity were formed after 10 mA/10 h of electrophoresis treatment. Furthermore, the calcium-phosphate ratio was similar to thermodynamically stable HA. In the synthesized biomaterial with addition of 1.0 wt% of GO, the colony forming units test showed significantly less Staphylococcus aureus. Initial attachment of MC3T3-E1 cells on the synthesized biomaterial was observed which showed the safety of the synthesized biomaterial for cell viability. This study showed that the synthesized biomaterial is a promising material that can be used in BTE.
各种材料都被应用于骨组织工程（BTE）。氧化石墨烯（GO）由于其抗菌活性和生物相容性，是 BTE 的良好候选材料。在这项研究中，我们使用电泳系统合成了一种由 GO、琼脂糖和羟基磷灰石（HA）组成的创新型生物材料。对合成生物材料的表征表明，经过 10 mA/10 h 的电泳处理后，形成了具有高纯度的针状晶体。此外，钙磷比与热力学稳定的 HA 相似。在添加 1.0 wt% GO 的合成生物材料中，集落形成单位试验表明金黄色葡萄球菌的数量明显减少。MC3T3-E1 细胞在合成生物材料上的初始附着表明该合成生物材料对细胞活力是安全的。本研究表明，所合成的生物材料是一种很有前途的材料，可以用于骨组织工程。
