Prakash J, Prema D, Venkataprasanna K S, Balagangadharan K, Selvamurugan N, Venkatasubbu G Devanand
Department of Nanotechnology, SRM Institute of Science and Technology, Kattankulathur, Kancheepuram Dist, Tamil Nadu, India.
Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur 603203, Tamil Nadu, India.
Int J Biol Macromol. 2020 Jul 1;154:62-71. doi: 10.1016/j.ijbiomac.2020.03.095. Epub 2020 Mar 12.
Recently, polymer based biomaterials are utilized in medical fields including surgical sutures, drug delivery devices, tissue supports and implants for interior bone fixation. However, polymer based implants leads to the formation of bio-films that are highly susceptible to microbial adhesion. In this study, we have fabricated Chitosan/Polyvinyl alcohol/Graphene oxide/Hydroxyapatite/gold films for potential orthopedic application. Graphene oxide/Hydroxyapatite/gold nanocomposite (GO/HAP/Au) was synthesized by simple hydrothermal method and GO/HAP/Au nanocomposite incorporated polymeric film was fabricated using gel casting method. The morphology, phase composition, crystalline structure and chemical state of the nanocomposite were characterized using as XRD, HR-TEM, FE-SEM and FT-IR. The bio-films were found to be biocompatible with mouse mesenchymal cells and it enhanced osteoblast differentiation as evidenced by more alkaline phosphatase activity at the cellular level. Hence, these results suggested that the developed nanocomposites films are osteogenic potential for treating bone and bone-related diseases. Antibacterial analysis of the films shows high inhibition zones against Gram positive and Gram Negative bacteria (Escherichia coli, streptococcus mutans, Staphylococcus aureus and Pseudomonas aeruginosa). Thus, the obtained nanocomposites bio-films are highly biocompatible and it can be used for bone regeneration application.
最近，基于聚合物的生物材料被应用于医学领域，包括手术缝线、药物递送装置、组织支架以及用于内部骨固定的植入物。然而，基于聚合物的植入物会导致生物膜的形成，这些生物膜极易受到微生物粘附的影响。在本研究中，我们制备了壳聚糖/聚乙烯醇/氧化石墨烯/羟基磷灰石/金薄膜，用于潜在的骨科应用。通过简单的水热法合成了氧化石墨烯/羟基磷灰石/金纳米复合材料（GO/HAP/Au），并采用凝胶浇铸法制备了包含GO/HAP/Au纳米复合材料的聚合物薄膜。使用XRD、HR-TEM、FE-SEM和FT-IR对纳米复合材料的形态、相组成、晶体结构和化学状态进行了表征。发现这些生物膜与小鼠间充质细胞具有生物相容性，并且在细胞水平上通过更多的碱性磷酸酶活性证明其增强了成骨细胞分化。因此，这些结果表明所开发的纳米复合薄膜具有治疗骨和骨相关疾病的成骨潜力。对薄膜的抗菌分析显示对革兰氏阳性和革兰氏阴性细菌（大肠杆菌、变形链球菌、金黄色葡萄球菌和铜绿假单胞菌）有高抑制区。因此，所获得的纳米复合生物膜具有高度生物相容性，可用于骨再生应用。
