Zhang He, Jiang Chunji, Lei Jingna, Dong Jiale, Ren Jingyao, Shi Xiaolong, Zhong Chao, Wang Xiaoguang, Zhao Xinhua, Yu Haiqiu
Peanut Research Institute, College of Agronomy, Shenyang Agricultural University, Shenyang 110161, China.
Peanut Research Institute, College of Agronomy, Shenyang Agricultural University, Shenyang 110161, China.
Genomics. 2022 Mar;114(2):110285. doi: 10.1016/j.ygeno.2022.110285. Epub 2022 Feb 4.
The unclear molecular mechanism by which peanuts adapt to chilling stress limits progress in molecular breeding for peanut chilling tolerance. Here, the physiological and transcriptional differences between two genotypes with contrasting tolerance under chilling stress were compared. The inhibition of photosynthesis mainly caused by stomatal factors was a common response of peanut seedlings to chilling stress. Chilling-tolerant genotypes could inhibit the accumulation of ROS to adapt to chilling stress, and enhanced activities of CAT and APX were major causes of lower HO content. The results of a conjoint analysis of physiological indices and the RNA-Seq database by WGCNA indicated that the genes in key modules were significantly enriched in pathways related to the oxidation-reduction process. Hub genes encoding RLK, CAT, MYC4, AOS, GST, PP2C, UPL5 and ZFP8 were likely to positively regulate peanut chilling tolerance, but hub genes encoding PAO, NAC2 and NAC72 were likely to negatively regulate peanut chilling tolerance.
花生适应低温胁迫的分子机制尚不清楚，这限制了花生耐低温分子育种的进展。在此，比较了两种在低温胁迫下耐受性不同的基因型之间的生理和转录差异。气孔因素导致的光合作用抑制是花生幼苗对低温胁迫的常见反应。耐低温基因型可以抑制活性氧的积累以适应低温胁迫，而CAT和APX活性的增强是HO含量降低的主要原因。通过WGCNA对生理指标和RNA-Seq数据库进行联合分析的结果表明，关键模块中的基因在与氧化还原过程相关的途径中显著富集。编码RLK、CAT、MYC4、AOS、GST、PP2C、UPL5和ZFP8的枢纽基因可能正向调节花生的耐低温性，而编码PAO、NAC2和NAC72的枢纽基因可能负向调节花生的耐低温性。
