通过不同低频电磁场能量对 Pax7、MyoD 和肌生成素的调节来调控 C2C12 细胞的成肌生成。

Regulation of skeletal myogenesis in C2C12 cells through modulation of Pax7, MyoD, and myogenin via different low-frequency electromagnetic field energies.

机构信息

Harbin Children's Hospital, Harbin, Heilongjiang, China.

Emergency Department, SongBei Hospital of The Fourth Hospital Affiliated of Harbin Medical University, Harbin, Heilongjiang, China.

出版信息

Technol Health Care. 2022;30(S1):371-382. doi: 10.3233/THC-THC228034.

DOI:10.3233/THC-THC228034

PMID:35124612

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9028610/

Abstract

BACKGROUND

A low-frequency electromagnetic field (LF-EMF) exerts important biological effects on the human body.

OBJECTIVE

We previously studied the immunity and atrophy of gastrocnemius muscles in rats with spinal cord injuries and found that LF-EMF with a magnetic flux density of 1.5 mT exerted excellent therapeutic and preventive effects on reducing myotubes and increasing spatium intermusculare. However, the effects of LF-EMF on all stages of skeletal myogenesis, such as activation, proliferation, differentiation, and fusion of satellite cells to myotubes as stimulated by myogenic regulatoryfactors (MRFs), have not been fully elucidated.

METHODS

This study investigated the optimal LF-EMF magnetic flux density that exerted maximal effects on all stages of C2C12 cell skeletal myogenesis as well as its impact on regulatory MRFs.

RESULTS

The results showed that an LF-EMF with a magnetic flux density of 2.0 mT could activate C2C12 cells and upregulate the proliferation-promoting transcription factor PAX7. On the other hand, 1.5 mT EMF could upregulate the expression of MyoD and myogenin.

CONCLUSION

LF-EMF could prevent the disappearance of myotubes, with different magnetic flux densities of LF-EMF exerting independent and positive effects on skeletal myogenesis such as satellite cell activation and proliferation, muscle cell differentiation, and myocyte fusion.

摘要