Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan, China.
Shenzhen Key Laboratory of Marine Bioresource and Eco-Environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, China.
Front Immunol. 2022 Jan 11;12:797096. doi: 10.3389/fimmu.2021.797096. eCollection 2021.
Acute liver injury (ALI) is a life-threatening syndrome with high mortality and lacks effective therapies. Rodents under LPS (lipopolysaccharide)/D-Gal (D-galactosamine) stress mimic ALI by presenting dramatically increased inflammation and cell death in the liver. , functioning like dietary fiber, is commonly used as a paramylon (Pa)-rich nutritional supplement that has various biological effects such as regulating immune system, anti-obesity, and anti-tumor. Here, we found that Pa or sonicated and alkalized paramylon (SA-Pa) alleviated the LPS/D-Gal-induced hepatic histopathological abnormalities in mice. Compared with Pa, SA-Pa had lower molecular weights/sizes and showed better efficacy in alleviating injury-induced hepatic functions, as well as the transcriptional levels of inflammatory cytokines. Moreover, SA-Pa treatment promoted M2 macrophage activation that enhanced the anti-inflammatory function in the liver, and downregulated STAT3 target genes, such as , and upon the injury. Meanwhile, SA-Pa treatment also alleviated apoptosis and necroptosis caused by the injury. Our results demonstrated that SA-Pa efficiently protected the liver from LPS/D-Gal-induced ALI by alleviating inflammation and cell death.
急性肝损伤（ALI）是一种具有高死亡率的危及生命的综合征，缺乏有效的治疗方法。LPS（脂多糖）/D-Gal（D-半乳糖）应激下的啮齿动物通过在肝脏中表现出明显增加的炎症和细胞死亡来模拟 ALI。作为膳食纤维，具有多种生物学作用，如调节免疫系统、抗肥胖和抗肿瘤等，常被用作富含拟南芥多糖（Pa）的营养补充剂。在这里，我们发现 Pa 或超声和碱化拟南芥（SA-Pa）可减轻 LPS/D-Gal 诱导的小鼠肝组织病理学异常。与 Pa 相比，SA-Pa 的分子量/尺寸更小，在缓解损伤诱导的肝功能以及炎症细胞因子的转录水平方面表现出更好的疗效。此外，SA-Pa 处理促进了 M2 巨噬细胞的激活，增强了肝脏的抗炎功能，并下调了 STAT3 靶基因，如在损伤时的 和 。同时，SA-Pa 处理还减轻了损伤引起的细胞凋亡和坏死性凋亡。我们的结果表明，SA-Pa 通过减轻炎症和细胞死亡，有效地保护肝脏免受 LPS/D-Gal 诱导的 ALI。
