General Surgery, Affiliated Hospital of Hebei University of Engineering, Handan 056002, Hebei, China.
Medical College, Hebei University of Engineering, Handan 056002, Hebei, China.
J Healthc Eng. 2022 Jan 25;2022:6280265. doi: 10.1155/2022/6280265. eCollection 2022.
polysaccharide (LBP) is the main active component of and has many beneficial effects, including neuroprotection, antiaging, and antioxidation. This study mainly explores the immunomodulatory effect of polysaccharides against liver fibrosis based on the intelligent medical Internet of Things. This measure emphasizes that the current effective methods and methods for the treatment of liver cancer are mainly combined treatments of Western medicine and Chinese medicine. These treatments have a certain effect in preventing liver cancer, reducing recurrence, and reducing side effects. Among them, chemotherapy has unique advantages in improving the quality of life and prolonging survival. With the development of medical science and technology, the clinical efficacy and efficacy of traditional Chinese medicine in the treatment of liver cancer are constantly improving. The mechanism is also studied from many aspects. The treatment time of LBPs on fibrotic hepatocytes was set to 24 h. Take liver fiber cells in logarithmic growth phase and incubate them at 37°C for 24 h. The whole process uses a temperature sensor for intelligent temperature control. In the experiment, groups of LBPs with different concentrations and different molecular weight ranges were set up and each group had 6 multiple holes. The original medium was aspirated and replaced with a medium containing different concentrations of LBPs (12.5, 25, 50, 100, and 200 g/mL) and cultured for 24 h. Based on the previous research, this study used in vitro cell experiments, microscopic observation, and MTT method to verify whether polysaccharides inhibit the proliferation of human liver cancer cells in vitro and whether they cooperate with the chemotherapy drug fluorouracil to play a tumor-killing effect. Animal experiments, using ELISA, HE staining, and other methods, explore the molecular and immunological mechanisms of LBP's antiliver cancer effect from the perspective of Th/Th2 differentiation balance and DC function, in order to provide experimental evidence for Chinese medicine polysaccharides in cancer immunotherapy and application. At different LBP concentrations (0 mol/L, 5 mol/L, 10 mol/L, and 15 mol/L), the inhibition rates were 0.80%, 20.06%, 35.44%, and 55.39%, respectively. This study provides a new method for large-scale expansion of hepatocytes in vitro, laying a stronger foundation for biological treatment of liver fibrosis.
多糖（LBP）是 的主要活性成分，具有许多有益的作用，包括神经保护、抗衰老和抗氧化作用。本研究主要基于智能医学物联网探索多糖对肝纤维化的免疫调节作用。该措施强调，目前治疗肝癌的有效方法和方法主要是中西医结合治疗。这些治疗方法在预防肝癌、降低复发率和减少副作用方面有一定的效果。其中，化疗在提高生活质量和延长生存时间方面具有独特的优势。随着医学科学技术的发展，中药在治疗肝癌中的临床疗效和疗效不断提高。其机制也从多方面进行了研究。LBPs 对纤维样肝细胞的治疗时间设定为 24 h。取对数生长期的肝纤维细胞，在 37°C 下孵育 24 h。整个过程采用温度传感器进行智能温度控制。在实验中，设置了不同浓度和不同分子量范围的 LBPs 组，每组有 6 个复孔。吸去原培养液，更换含不同浓度 LBPs（12.5、25、50、100 和 200μg/ml）的培养液，培养 24 h。基于前期研究，本研究采用体外细胞实验、显微镜观察和 MTT 法，验证 多糖是否能抑制人肝癌细胞的体外增殖，并与化疗药物氟尿嘧啶协同发挥杀伤肿瘤作用。动物实验采用 ELISA、HE 染色等方法，从 Th/Th2 分化平衡和 DC 功能的角度探讨 LBP 抗肝癌作用的分子和免疫学机制，为中药多糖在癌症免疫治疗和应用中提供实验依据。在不同 LBP 浓度（0mol/L、5mol/L、10mol/L 和 15mol/L）下，抑制率分别为 0.80%、20.06%、35.44%和 55.39%。本研究为体外大规模扩增肝细胞提供了一种新方法，为肝纤维化的生物治疗奠定了更坚实的基础。
