Valderrama Andrés, Zapata Maria Isabel, Hernandez Juan C, Cardona-Arias Jaiberth A
Infettare, Facultad de Medicina, Universidad Cooperativa de Colombia, Colombia.
Escuela de Microbiología, University of Antioquia, Colombia.
Heliyon. 2022 Jan 25;8(1):e08778. doi: 10.1016/j.heliyon.2022.e08778. eCollection 2022 Jan.
Preclinical evidence about the neutrophil-mediated response in exposure to air pollutants is scattered and heterogeneous. This has prevented the consolidation of this research field around relevant models that could advance towards clinical research. The purpose of this study was to systematic review the studies of the neutrophils response to air pollutants, following the recommendations of the Cochrane Collaboration and the PRISMA guide, through 54 search strategies in nine databases. We include 234 studies (, and ), being more frequent using primary neutrophils, Balb/C and C57BL6/J mice, and Sprague-Dawley and Wistar rats. The most frequent readouts were cell counts, cytokines and histopathology. The temporal analysis showed that in the last decade, the use of mice with histopathological and cytokine measurement have predominated. This systematic review has shown that study of the neutrophils response to air pollutants started 40 years ago, and composed of 100 different preclinical models, 10 pollutants, and 11 immunological outcomes. Mechanisms of neutrophils-mediated immunopathology include cellular activation, ROS production, and proinflammatory effects, leading to cell-death, oxidative stress, and inflammatory infiltrates in lungs. This research will allow consolidating the research efforts in this field, optimizing the study of causal processes, and facilitating the advance to clinical studies.
关于暴露于空气污染物中中性粒细胞介导反应的临床前证据零散且不统一。这阻碍了围绕相关模型巩固该研究领域，而这些模型本可推动临床研究发展。本研究的目的是按照Cochrane协作网和PRISMA指南的建议，通过在九个数据库中采用54种检索策略，系统回顾关于中性粒细胞对空气污染物反应的研究。我们纳入了234项研究（……），使用原代中性粒细胞、Balb/C和C57BL6/J小鼠以及Sprague-Dawley和Wistar大鼠的情况更为常见。最常见的检测指标是细胞计数、细胞因子和组织病理学。时间分析表明，在过去十年中，使用小鼠进行组织病理学和细胞因子测量的研究占主导地位。这项系统综述表明，对中性粒细胞对空气污染物反应的研究始于40年前，由100种不同的临床前模型、10种污染物和11种免疫学结果组成。中性粒细胞介导的免疫病理学机制包括细胞活化、活性氧生成和促炎作用，导致细胞死亡、氧化应激和肺部炎症浸润。这项研究将有助于巩固该领域的研究工作，优化因果过程的研究，并促进向临床研究的推进。
