Institute of Functional Molecules, Shenyang University of Chemical Technology, Shenyang, PR China.
Liaoning Province Key Laboratory of Green Functional Molecular Design and Development, Shenyang University of Chemical Technology, Shenyang, PR China.
J Food Biochem. 2022 May;46(5):e14068. doi: 10.1111/jfbc.14068. Epub 2022 Feb 7.
Neurodegenerative diseases (NDDs) are chronic neurological disorders associated with cognitive or motor dysfunction. As a common spice, Zingiber officinale Roscoe has been used as a medicine to treat a variety of NDDs. However, at the molecular level, the mechanisms of Z. officinale in treating of NDDs have not been deeply investigated. In this study, network pharmacology method, molecular docking, Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were used to predict the mechanisms of Z. officinale in the treatment of NDDs. After a series of biological information analyses, five core targets were obtained, including heme oxygenase 1 (HMOX1), acetylcholinesterase (AChE), nitric oxide synthase (NOS), catechol-O-methyl-transferase (COMT), and metabotropic glutamate receptor 5 (mGluR5). Compounds 75, 68, 46, 67, 69, 49, 66, 50, 34, and 64 were identified as the main components of Z. officinale in the treatment of NDDs. The crucial pathways mainly include neuroactive ligand-receptor signaling pathways, cyclic adenosine monophosphate signaling pathways, dopamine synaptic signaling pathways, and so on. Besides, in vitro experiments by AChE inhibitory activities assay and neuroprotective activities against H O -induced injury in human neuroblastoma SH-SY5Y cells validated the reliability of the results of network analysis. PRACTICAL APPLICATIONS: Zingiber officinale Roscoe is widely used as a traditional spice and herbal medicine. It contains a number of active ingredients, which have shown activities on anti-neurodegenerative diseases (NDDs). In this paper, the potential mechanism of Z. officinale in the treatment of NDDs is explored through network pharmacology, and it was verified by in vitro experiments. The mechanism was not only clarified at the system level but also proved to be effective at the biological level. The results can be used as a reference for Z. officinale in the treating of NDDs.
神经退行性疾病（NDDs）是与认知或运动功能障碍相关的慢性神经紊乱。作为一种常见的香料，姜黄已被用作治疗各种 NDDs 的药物。然而，在分子水平上，姜黄治疗 NDDs 的机制尚未得到深入研究。在这项研究中，使用网络药理学方法、分子对接、基因本体论（GO）分析和京都基因与基因组百科全书（KEGG）富集分析来预测姜黄治疗 NDDs 的机制。经过一系列生物信息分析，获得了五个核心靶点，包括血红素加氧酶 1（HMOX1）、乙酰胆碱酯酶（AChE）、一氧化氮合酶（NOS）、儿茶酚-O-甲基转移酶（COMT）和代谢型谷氨酸受体 5（mGluR5）。鉴定出化合物 75、68、46、67、69、49、66、50、34 和 64 为姜黄治疗 NDDs 的主要成分。关键途径主要包括神经活性配体-受体信号通路、环磷酸腺苷信号通路、多巴胺突触信号通路等。此外，通过 AChE 抑制活性测定和体外人神经母细胞瘤 SH-SY5Y 细胞中 HO 诱导损伤的神经保护活性实验验证了网络分析结果的可靠性。实际应用：姜黄是一种广泛用作传统香料和草药的植物。它含有多种活性成分，对神经退行性疾病（NDDs）具有活性。本文通过网络药理学探讨了姜黄治疗 NDDs 的潜在机制，并通过体外实验进行了验证。该机制不仅在系统水平上得到了阐明，而且在生物水平上也得到了证实。该结果可以为姜黄治疗 NDDs 提供参考。
