IGF2BP2 可能是口腔鳞状细胞癌的一种新型预后生物标志物。
IGF2BP2 maybe a novel prognostic biomarker in oral squamous cell carcinoma.
机构信息
Department of Oral and Maxillofacial Plastic and Trauma Surgery, Center of Cleft Lip and Palate Treatment, Beijing Stomatological Hospital, Capital Medical University, Beijing, China.
Department of Oral and Maxillofacial and Head and Neck Oncology, Beijing Stomatological Hospital, Capital Medical University, Beijing, China.
出版信息
Biosci Rep. 2022 Feb 25;42(2). doi: 10.1042/BSR20212119.
AIM
The main of the present study was to investigate the role of insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) in oral squamous cell carcinoma (OSCC) with the overarching of providing new biomarkers or potential therapeutic targets for OSCC.
METHODS
We combined datasets downloaded from Gene Expression Omnibus (GEO), The Cancer Genome Atlas (TCGA), and samples collected from the clinic to evaluate the expression of IGF2BP2 in OSCC. IGF2BP2 survival analysis was respectively performed based on TCGA, GEO, and clinical samples. Correlations between IGF2BP2 expression and clinicopathological parameters were then analyzed, and signaling pathways associated with IGF2BP2 expression were identified using gene set enrichment analysis (GSEA 4.1.0). Moreover, an IGF2BP2 co-expressed gene network was constructed, followed by gene ontology (GO) functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis on IGF2BP2 co-expressed genes. Finally, TIMER and CIBERSORT were used to analyze the correlations among IGF2BP2, IGF2BP2-coexpressed genes, and tumor-infiltrating immune cells (TICs).
RESULTS
IGF2BP2 was highly expressed in OSCC and significantly correlated with overall survival of OSCC patients (P<0.01). High IGF2BP2 expression correlated with poor overall survival. The GSEA results showed that cell apoptosis-, tumor-, and immune-related pathways were significantly enriched in samples with high IGF2BP2 expression. Furthermore, GO and KEGG enrichment analyses results of IGF2BP2 co-expressed genes indicated that these genes are mainly associated with immunity/inflammation and tumorigenesis. In addition, IGF2BP2 and its co-expressed genes are associated with TICs (P<0.01).
CONCLUSION
IGF2BP2 may be a potential prognostic biomarker in OSCC and correlates with immune infiltrates.
目的
本研究的主要目的是探讨胰岛素样生长因子 2 mRNA 结合蛋白 2（IGF2BP2）在口腔鳞状细胞癌（OSCC）中的作用，为 OSCC 提供新的生物标志物或潜在的治疗靶点。
方法
我们结合从基因表达综合数据库（GEO）、癌症基因组图谱（TCGA）下载的数据集和从临床收集的样本，评估 IGF2BP2 在 OSCC 中的表达。分别基于 TCGA、GEO 和临床样本进行 IGF2BP2 生存分析。然后分析 IGF2BP2 表达与临床病理参数的相关性，并使用基因集富集分析（GSEA 4.1.0）鉴定与 IGF2BP2 表达相关的信号通路。此外，构建 IGF2BP2 共表达基因网络，对 IGF2BP2 共表达基因进行基因本体（GO）功能富集分析和京都基因与基因组百科全书（KEGG）通路富集分析。最后，使用 TIMER 和 CIBERSORT 分析 IGF2BP2、IGF2BP2 共表达基因与肿瘤浸润免疫细胞（TICs）之间的相关性。
结果
IGF2BP2 在 OSCC 中高表达，与 OSCC 患者的总生存率显著相关（P<0.01）。高 IGF2BP2 表达与总生存率差相关。GSEA 结果表明，在 IGF2BP2 高表达的样本中，细胞凋亡、肿瘤和免疫相关途径显著富集。此外，IGF2BP2 共表达基因的 GO 和 KEGG 富集分析结果表明，这些基因主要与免疫/炎症和肿瘤发生有关。此外，IGF2BP2 及其共表达基因与 TICs 相关（P<0.01）。
结论
IGF2BP2 可能是 OSCC 潜在的预后生物标志物，与免疫浸润相关。
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