Liao Bo, Xu Cheng, Wang Zhenglun, Li Wei, Liu Xinyu, Lu Dong
Key Laboratory for Advanced Technology of Materials of Ministry of Education, Tribology Research Institute, Southwest Jiaotong University, Chengdu, 610031, China.
Chengdu Advanced Metal Materials Industry Technology Research Institute, Chengdu, 610300, China.
J Bone Miner Metab. 2022 May;40(3):402-414. doi: 10.1007/s00774-022-01309-7. Epub 2022 Feb 7.
Bacterial infection and aseptic loosening caused by bone resorption at the implant interface are major clinical complications during bone defect implantation surgery, and surface modification of the implant to address the aforementioned problems has long been a research focus.
In this paper, a chitosan (CTS)-tannic acid (TA) colloid coating with a negative charge and excellent hydrophilicity was prepared on a Ti6Al4V (TC4) surface using a layer-by-layer assembly method. The physical properties, anti-osteoclast activity, and antimicrobial activity of the coatings were investigated.
The findings showed that when the pH value was 5 and the ratio of CTS:TA was 0.8, the carrying rate of TA was the best. Furthermore, the CTS-TA coating had no cytotoxicity on the morphology and proliferation of BMSCs cells and effectively inhibited the differentiation of RAW264.7 cells into osteoclasts and the proliferation of Staphylococcus aureus and Escherichia coli. With the increase in the immersion time of TC4 in CTS-TA colloid solution, the inhibitory effects will also enhance.
Therefore, the preparation of the CTS-TA coating provides a revolutionary technique for implant surface modification to avoid postoperative bacterial infection and aseptic loosening.
植入界面处的骨吸收导致的细菌感染和无菌性松动是骨缺损植入手术中的主要临床并发症，通过对植入物进行表面改性来解决上述问题一直是研究热点。
本文采用层层组装法在Ti6Al4V（TC4）表面制备了具有负电荷和优异亲水性的壳聚糖（CTS）-单宁酸（TA）胶体涂层。对涂层的物理性能、抗破骨细胞活性和抗菌活性进行了研究。
研究结果表明，当pH值为5且CTS:TA比例为0.8时，TA的负载率最佳。此外，CTS-TA涂层对骨髓间充质干细胞（BMSCs）的形态和增殖无细胞毒性，并能有效抑制RAW264.7细胞向破骨细胞的分化以及金黄色葡萄球菌和大肠杆菌的增殖。随着TC4在CTS-TA胶体溶液中浸泡时间的增加，抑制效果也会增强。
因此，CTS-TA涂层的制备为植入物表面改性提供了一种创新性技术，以避免术后细菌感染和无菌性松动。
