Harvey R P, Degryse E, Stefani L, Schamber F, Cazenave J P, Courtney M, Tolstoshev P, Lecocq J P
Proc Natl Acad Sci U S A. 1986 Feb;83(4):1084-8. doi: 10.1073/pnas.83.4.1084.
Cloned cDNAs have been isolated that encode a variant of hirudin, a potent thrombin inhibitor that is secreted by the salivary glands of the medicinal leech, Hirudo medicinalis. This variant probably corresponds to a form that has been purified from leech heads but differs in amino acid sequence from the hirudin purified from whole leeches. There are at least three hirudin transcripts detectable in leech RNAs that are different in size, site of synthesis, inducibility by starvation, and relationship to hirudin activity. The new hirudin variant predicted by the cDNA and the heterodisperse transcription products suggest a hirudin protein family. The hirudin cDNA was expressed in Escherichia coli under the control of the bacteriophage lambda PL promoter. The recombinant product is biologically active, inhibiting the cleavage by thrombin of fibrinogen and a synthetic tripeptide substrate.
已分离出克隆的互补脱氧核糖核酸（cDNA），其编码水蛭素的一种变体。水蛭素是一种强效凝血酶抑制剂，由药用蚂蟥（医用水蛭）的唾液腺分泌。这种变体可能对应于一种已从水蛭头部纯化出来的形式，但氨基酸序列与从整个水蛭中纯化出的水蛭素不同。在水蛭RNA中可检测到至少三种水蛭素转录本，它们在大小、合成位点、饥饿诱导性以及与水蛭素活性的关系方面存在差异。由cDNA预测的新水蛭素变体和异源分散的转录产物提示存在一个水蛭素蛋白家族。水蛭素cDNA在噬菌体λ PL启动子的控制下在大肠杆菌中表达。重组产物具有生物活性，可抑制凝血酶对纤维蛋白原和一种合成三肽底物的切割。
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