Sauvage Center for Molecular Sciences, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China.
Angew Chem Int Ed Engl. 2022 Apr 4;61(15):e202115820. doi: 10.1002/anie.202115820. Epub 2022 Feb 18.
The current strategies for nanoelectrode functionalization usually involve sophisticated modification procedures, uncontrollable and unstable modifier assembly, as well as a limited variety of modifiers. To address this issue, we propose a versatile strategy for large-scale synthesis of biomimetic molecular catalysts (BMCs) modified nanowires (NWs) to construct functionalized electrochemical nanosensors. This design protocol employs an easy, controllable and stable assembly of diverse BMCs-poly(3,4-ethylenedioxythiophene) (PEDOT) composites on conductive NWs. The intrinsic catalytic activity of BMCs combined with outstanding electron transfer ability of conductive polymer enables the nanosensors to sensitively and selectively detect various biomolecules. Further application of sulfonated cobalt phthalocyanine functionalized nanosensors achieves real-time electrochemical monitoring of intracellular glutathione levels and its redox homeostasis in single living cells for the first time.
目前纳米电极功能化的策略通常涉及复杂的修饰程序、不可控和不稳定的修饰剂组装，以及有限的修饰剂种类。为了解决这个问题，我们提出了一种通用的策略，用于大规模合成仿生分子催化剂（BMCs）修饰的纳米线（NWs），以构建功能化的电化学纳米传感器。该设计方案采用一种简便、可控和稳定的方法，将各种 BMCs-聚（3,4-乙撑二氧噻吩）（PEDOT）复合材料组装在导电 NWs 上。BMCs 的内在催化活性和导电聚合物的出色电子转移能力使纳米传感器能够灵敏和选择性地检测各种生物分子。进一步应用磺化钴酞菁功能化的纳米传感器，首次实现了对单个活细胞内谷胱甘肽水平及其氧化还原动态平衡的实时电化学监测。
