一种名为 AsES 的真菌蛋白酶触发抗病毒免疫反应，并有效地限制了拟南芥和本氏烟植物中的病毒感染。
A fungal protease named AsES triggers antiviral immune responses and effectively restricts virus infection in arabidopsis and Nicotiana benthamiana plants.
机构信息
Instituto de Agrobiotecnología y Biología Molecular (IABIMO), CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), De los Reseros y N. Repetto s/n, Hurlingham B1686IGC, Argentina.
Estación Experimental Agropecuaria Famaillá, Instituto Nacional de Tecnología Agropecuaria (INTA), Ruta Provincial 301 Km 32, Tucumán, Argentina.
出版信息
Ann Bot. 2022 Apr 13;129(5):593-606. doi: 10.1093/aob/mcac013.
BACKGROUND AND AIMS
Plants have evolved complex mechanisms to fight against pathogens. Among these mechanisms, pattern-triggered immunity (PTI) relies on the recognition of conserved microbe- or pathogen-associated molecular patterns (MAMPs or PAMPs, respectively) by membrane-bound receptors. Indeed, PTI restricts virus infection in plants and, in addition, BRI1-associated kinase 1 (BAK1), a central regulator of PTI, plays a role in antiviral resistance. However, the compounds that trigger antiviral defences, along with their molecular mechanisms of action, remain mostly elusive. Herein, we explore the role of a fungal extracellular subtilase named AsES in its capacity to trigger antiviral responses.
METHODS
In this study, we obtained AsES by recombinant expression, and evaluated and characterized its capacity to trigger antiviral responses against Tobacco mosaic virus (TMV) by performing time course experiments, analysing gene expression, virus movement and callose deposition.
KEY RESULTS
The results of this study provide direct evidence that exogenous treatment with recombinant AsES increases a state of resistance against TMV infection, in both arabidopsis and Nicotiana benthamiana plants. Also, the antiviral PTI response exhibited by AsES in arabidopsis is mediated by the BAK1/SERK3 and BKK1/SERK4 co-receptors. Moreover, AsES requires a fully active salicylic acid (SA) signalling pathway to restrict the TMV movement by inducing callose deposition. Additionally, treatment with PSP1, a biostimulant based on AsES as the active compound, showed an increased resistance against TMV in N. benthamiana and tobacco plants.
CONCLUSIONS
AsES is a fungal serine protease which triggers antiviral responses relying on a conserved mechanism by means of the SA signalling pathway and could be exploited as an effective and sustainable biotechnology strategy for viral disease management in plants.
背景与目的
植物已经进化出复杂的机制来对抗病原体。在这些机制中，模式触发免疫（PTI）依赖于膜结合受体识别保守的微生物或病原体相关分子模式（MAMPs 或 PAMPs）。事实上，PTI 限制了植物中的病毒感染，此外，作为 PTI 的中央调节剂的 BRASSINOSTEROID-INSENSITIVE 1-ASSOCIATED KINASE 1（BAK1）在抗病毒抗性中也发挥作用。然而，触发抗病毒防御的化合物及其作用的分子机制仍然大多难以捉摸。在此，我们探索了一种名为 AsES 的真菌细胞外枯草杆菌蛋白酶在触发抗病毒反应中的作用。
方法
在本研究中，我们通过重组表达获得了 AsES，并通过进行时间进程实验、分析基因表达、病毒运动和胼胝质沉积来评估和表征其触发针对烟草花叶病毒（TMV）的抗病毒反应的能力。
主要结果
本研究的结果提供了直接证据，表明外源性重组 AsES 处理可增加拟南芥和烟草原生质体植物对 TMV 感染的抗性状态。此外，AsES 在拟南芥中诱导的抗病毒 PTI 反应由 BAK1/SERK3 和 BKK1/SERK4 共受体介导。此外，AsES 需要一个完全活跃的水杨酸（SA）信号通路来通过诱导胼胝质沉积来限制 TMV 的运动。此外，用 PSP1（一种基于 AsES 作为活性化合物的生物刺激素）处理可提高烟草原生质体和烟草植物对 TMV 的抗性。
结论
AsES 是一种真菌丝氨酸蛋白酶，它通过依赖于 SA 信号通路的保守机制触发抗病毒反应，可被用作管理植物病毒病的有效和可持续的生物技术策略。
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