Sekizawa J, Shibamoto T
Mutat Res. 1982 Apr;101(2):127-40. doi: 10.1016/0165-1218(82)90003-9.
The genotoxicity of safrole, 9 compounds that are structurally similar to safrole (anethole, cinnamaldehyde, cinnamyl alcohol, estragole, methyl eugenol, eugenol, isoeugenol, isosafrole, piperonal), 5 essential oils, cassia oil, cinnamon bark oil, clove oil, fennel oil) which contain the chemicals tested, and 1 oleoresin was studies in 3 microbial test systems. Only anethole showed mutagenicity in the Ames Salmonella reversion assay. All chemicals except anethole, estragole and isosafrole were positive in the Bacillus subtilis DNA-repair test (Rec assay) without S9. All samples tested were negative in the Escherichia coli WP2 uvrA reversion test. The essential oils and pimenta oleoresin were positive in the DNA-repair test. The results obtained are discussed in relation to the nature of the problems encountered with each test method.
在3种微生物测试系统中研究了黄樟素、9种与黄樟素结构相似的化合物（茴香脑、肉桂醛、肉桂醇、草蒿脑、甲基丁香酚、丁香酚、异丁香酚、异黄樟素、胡椒醛）、5种含有受试化学物质的精油（桂皮油、肉桂皮油、丁香油、茴芹油）以及1种油树脂的遗传毒性。只有茴香脑在鼠伤寒沙门氏菌回复突变试验中显示出致突变性。除茴香脑、草蒿脑和异黄樟素外，所有化学物质在无S9的枯草芽孢杆菌DNA修复试验（Rec试验）中均呈阳性。所有测试样品在大肠杆菌WP2 uvrA回复突变试验中均为阴性。精油和多香果油树脂在DNA修复试验中呈阳性。结合每种测试方法遇到的问题的性质对所得结果进行了讨论。
