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Ro/SS-A抗原的细胞定位

Cellular localization of the Ro/SS-A antigen.

作者信息

Lopez-Robles E, Herrera-Esparza R, Avalos-Diaz E

出版信息

Clin Rheumatol. 1986 Jan;5(1):33-8. doi: 10.1007/BF02030965.

DOI:10.1007/BF02030965
PMID:3514080
Abstract

The cellular localization of the Ro/SS-A antigen was defined using indirect immunofluorescence and eight monospecific anti- Ro/SS-A antisera which were identified by immunoblotting. Several mammalian tissues were used as substrates. The Ro/SS-A antigen was located mainly in the nucleus of dog liver and Hep-2-cells, and anti-Ro sera had produced a speckled staining pattern. Cytoplasmic fluorescence appeared only in one serum. IgG was the predominant immunoglobulin with anti-Ro/SS-A activity; five sera had complement fixing activity. The sera absorption studies with partially purified Ro/SS-A antigen and the negativation of the ANA tests had confirmed the specificity of our findings.

摘要

利用间接免疫荧光法以及通过免疫印迹鉴定的8种单特异性抗Ro/SS - A抗血清，确定了Ro/SS - A抗原的细胞定位。使用了几种哺乳动物组织作为底物。Ro/SS - A抗原主要位于犬肝和Hep - 2细胞的细胞核中，抗Ro血清产生斑点状染色模式。仅在一种血清中出现细胞质荧光。IgG是具有抗Ro/SS - A活性的主要免疫球蛋白；5种血清具有补体固定活性。用部分纯化的Ro/SS - A抗原进行的血清吸收研究以及ANA试验的阴性结果证实了我们发现的特异性。

相似文献

1
Cellular localization of the Ro/SS-A antigen.Ro/SS-A抗原的细胞定位
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2
Strong Association of the Myriad Discrete Speckled Nuclear Pattern With Anti-SS-A/Ro60 Antibodies: Consensus Experience of Four International Expert Centers.众多离散斑点核型与抗-SS-A/Ro60 抗体的强烈关联：四个国际专家中心的共识经验。
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The importance of tissue substrate in the SS-A/Ro antigen-antibody system.组织底物在SS-A/Ro抗原抗体系统中的重要性。
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4
Detection of autoantibodies to SS-A/Ro by indirect immunofluorescence using a transfected and overexpressed human 60 kD Ro autoantigen in HEp-2 cells.使用在HEp-2细胞中经转染和过表达的人60 kD Ro自身抗原，通过间接免疫荧光法检测抗SS-A/Ro自身抗体。
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Routine immunofluorescence detection of Ro/SS-A autoantibody using HEp-2 cells transfected with human 60 kDa Ro/SS-A.使用转染了人60 kDa Ro/SS-A的HEp-2细胞进行Ro/SS-A自身抗体的常规免疫荧光检测。
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[Intracellular localization of the SS-A/Ro antigen--studies using cell fractionation and fluorescent antibody technics].[SS-A/Ro抗原的细胞内定位——运用细胞分级分离和荧光抗体技术的研究]
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引用本文的文献

1
Analysis of the intracellular localization and assembly of Ro ribonucleoprotein particles by microinjection into Xenopus laevis oocytes.通过显微注射到非洲爪蟾卵母细胞中来分析Ro核糖核蛋白颗粒的细胞内定位和组装。
J Cell Biol. 1994 Jun;125(5):981-8. doi: 10.1083/jcb.125.5.981.
2
Protein heterogeneity in the human Ro/SSA ribonucleoproteins. The 52- and 60-kD Ro/SSA autoantigens are encoded by separate genes.人类Ro/SSA核糖核蛋白中的蛋白质异质性。52-kD和60-kD Ro/SSA自身抗原由不同基因编码。
J Clin Invest. 1991 Jan;87(1):177-86. doi: 10.1172/JCI114968.
3
A human monoclonal autoantibody to a nucleolar structure.

本文引用的文献

1
Correlative histologic and serologic findings of sicca syndrome in patients with systemic lupus erythematosus.系统性红斑狼疮患者干燥综合征的相关组织学和血清学发现。
Arthritis Rheum. 1980 Jan;23(1):36-40. doi: 10.1002/art.1780230107.
2
Neonatal lupus erythematosus: new serologic findings.新生儿红斑狼疮：新的血清学发现。
J Invest Dermatol. 1981 Sep;77(3):331-3. doi: 10.1111/1523-1747.ep12482531.
3
A serological marker for neonatal lupus erythematosus.一种新生儿红斑狼疮的血清学标志物。
一种针对核仁结构的人源单克隆自身抗体。
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4
Connective-tissue disease, antibodies to ribonucleoprotein, and congenital heart block.结缔组织病、抗核糖核蛋白抗体与先天性心脏传导阻滞。
N Engl J Med. 1983 Jul 28;309(4):209-12. doi: 10.1056/NEJM198307283090403.
5
Placental transfer of anticytoplasmic antibodies in annular erythema of newborns.
Arch Dermatol. 1981 Sep;117(9):569-72.
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Partial immunochemical characterization of the Ro and La proteins using antibodies from patients with the sicca syndrome and lupus erythematosus.利用干燥综合征和红斑狼疮患者的抗体对Ro和La蛋白进行部分免疫化学特性分析。
J Immunol. 1984 May;132(5):2350-6.
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Systemic lupus erythematosus in the elderly: clinical and serological characteristics.老年系统性红斑狼疮：临床和血清学特征
J Rheumatol. 1984 Apr;11(2):175-81.
8
Homozygous C2 deficiency, lupus erythematosus, and anti-Ro (SSA) antibodies.纯合子C2缺乏、红斑狼疮和抗Ro（SSA）抗体。
Arthritis Rheum. 1983 Oct;26(10):1279-82. doi: 10.1002/art.1780261017.
9
The importance of tissue substrate in the SS-A/Ro antigen-antibody system.组织底物在SS-A/Ro抗原抗体系统中的重要性。
Arthritis Rheum. 1984 Feb;27(2):166-73. doi: 10.1002/art.1780270207.
10
The Ro small cytoplasmic ribonucleoproteins: identification of the antigenic protein and its binding site on the Ro RNAs.Ro小细胞质核糖核蛋白：抗原蛋白的鉴定及其在Ro RNA上的结合位点
Proc Natl Acad Sci U S A. 1984 Apr;81(7):1996-2000. doi: 10.1073/pnas.81.7.1996.