Zhang Chenghua, Deng Wangqiu, Yan Wenjuan, Li Taihui
State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou, 510070, China.
State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou, 510070, China
G3 (Bethesda). 2018 May 31;8(6):1863-1870. doi: 10.1534/g3.118.200287.
is an edible fungus which was approved as a novel food by the Chinese Ministry of Public Health in 2013. It also has a broad prospect of application in pharmaceutical industries, with many medicinal activities. In this study, the whole genome of GD15, a single spore isolate from a wild strain, was sequenced and assembled with Illumina and PacBio sequencing technology. The generated genome is 29.05 Mb in size, comprising nine scaffolds with an average GC content of 57.01%. It is predicted to contain a total of 9150 protein-coding genes. Sequence identification and comparative analysis indicated that the assembled scaffolds contained two complete chromosomes and four single-end chromosomes, showing a high level assembly. Gene annotation revealed a diversity of transposons that could contribute to the genome size and evolution. Besides, approximately 15.57% and 12.01% genes involved in metabolic processes were annotated by KEGG and COG respectively. Genes belonging to CAZymes accounted for 3.15% of the total genes. In addition, 435 transcription factors, involved in various biological processes, were identified. Among the identified transcription factors, the fungal transcription regulatory proteins (18.39%) and fungal-specific transcription factors (19.77%) represented the two largest classes of transcription factors. This genomic resource provided a new insight into better understanding the relevance of phenotypic characters and genetic mechanisms in .
是一种可食用真菌,于2013年被中国卫生部批准为新食品。它在制药行业也有广阔的应用前景,具有多种药用活性。在本研究中,对一株野生菌株的单孢分离株GD15的全基因组进行了测序,并采用Illumina和PacBio测序技术进行组装。生成的基因组大小为29.05 Mb,由9个支架组成,平均GC含量为57.01%。预计总共包含9150个蛋白质编码基因。序列鉴定和比较分析表明,组装的支架包含两条完整染色体和四条单端染色体,显示出高水平的组装。基因注释揭示了多种转座子,它们可能对基因组大小和进化有贡献。此外,分别有大约15.57%和12.01%参与代谢过程的基因被KEGG和COG注释。属于碳水化合物活性酶的基因占总基因的3.15%。此外,鉴定出435个参与各种生物过程的转录因子。在鉴定出的转录因子中,真菌转录调节蛋白(18.39%)和真菌特异性转录因子(19.77%)是两类最大的转录因子。这一基因组资源为更好地理解[具体物种]的表型特征与遗传机制之间的相关性提供了新的见解。