Mauch C, Aumailley M, Paye M, Lapière C M, Timpl R, Krieg T
Exp Cell Res. 1986 Apr;163(2):294-300. doi: 10.1016/0014-4827(86)90060-1.
Attachment of fibroblasts from dermatosparactic sheep and cattle to collagenous substrates (types I and IV) is defective (30-50%) when compared with fibroblasts from normal or heterozygous animals. The difference was independent of the amount of substrate, incubation time and protein synthesis. No differences were observed in the binding to fibronectin or laminin. Reduced attachment to collagen can be partially restored by adding fibronectin. The polygonal morphology of dermatosparactic cells was, however, not altered by attachment and growth on dishes coated with different collagens or fibronectin. Reduced interaction with collagens could be due to changes in specific receptors and may represent a further pathological change in dermatosparactic animals.
与正常或杂合动物的成纤维细胞相比，皮肤松垂症绵羊和牛的成纤维细胞与胶原底物（I型和IV型）的附着存在缺陷（30%-50%）。这种差异与底物量、孵育时间和蛋白质合成无关。在与纤连蛋白或层粘连蛋白的结合方面未观察到差异。通过添加纤连蛋白，对胶原的附着减少可得到部分恢复。然而，在涂有不同胶原或纤连蛋白的培养皿上附着和生长时，皮肤松垂症细胞的多边形形态并未改变。与胶原的相互作用减少可能是由于特定受体的变化，这可能代表皮肤松垂症动物的另一种病理变化。
