School of Applied Chemical Engineering, Kyungpook National University, 80, Daehak-ro, Buk-gu, Daegu, 41566, Republic of Korea; Department of Polymer Science & Engineering, Kyungpook National University, 80, Daehak-ro, Buk-gu, Daegu, 41566, Republic of Korea.
Department of Cogno-Mechatronics Engineering, Department of Optics and Mechatronics Engineering, College of Nanoscience and Nanotechnology, Pusan National University, Busan, 46241, Republic of Korea.
Biosens Bioelectron. 2022 May 15;204:114073. doi: 10.1016/j.bios.2022.114073. Epub 2022 Feb 3.
In this study, protein-imprinted sensors were electrochemically fabricated on screen-printed carbon electrodes (SPCEs) for the cytokine interleukin-1β (IL-1β) detection. A double layer comprising poly(o-phenylenediamine) and poly(chromotrope 2R) with a template (i.e., IL-1β biomacromolecules) was formed through the cyclic voltammetry (CV) technique to modify the molecularly imprinted polymer (MIP) films on the SPCEs. The electrochemical sensing properties were investigated via CV and electrochemical impedance spectroscopy to confirm the imprinting effect on the MIP films. The results show that the MIP sensor has a highly sensitive response in the trace IL-1β solution (a few pg/mL) with a limit of detection of 0.23 pg/mL and a limit of quantification of 0.78 pg/mL. Furthermore, the MIP sensor showed high selectivity for IL-1β adsorption compared to other proteins. In summary, based on binary double layers, the impedance sensing platforms of electropolymerized MIP films show potential application in the practical detection of macromolecular proteins.
在这项研究中，基于丝网印刷碳电极（SPCE）电化学制备了蛋白质印迹传感器，用于细胞因子白细胞介素-1β（IL-1β）的检测。通过循环伏安法（CV）技术在 SPCE 上形成包含聚邻苯二胺和聚变色酸 2R 的双层，其中包含模板（即 IL-1β 生物大分子），以修饰分子印迹聚合物（MIP）薄膜。通过 CV 和电化学阻抗谱研究了电化学传感特性，以确认 MIP 薄膜上的印迹效应。结果表明，MIP 传感器在痕量 IL-1β 溶液（几个 pg/mL）中具有高灵敏度响应，检测限为 0.23 pg/mL，定量限为 0.78 pg/mL。此外，与其他蛋白质相比，MIP 传感器对 IL-1β 的吸附表现出高选择性。总之，基于双二元层，电聚合 MIP 薄膜的阻抗传感平台显示出在实际检测大分子蛋白质方面的潜在应用。
