Roberts Tamalee, Silisouk Joy, Sengdatka Davanh, Sibounheuang Bountoy, Seljuk Ranoy, Vang Xao, Sengduangphachanh Amphonesavanh, Davong Viengmon, Vongsouvath Manivanh, Malou Nada, Ferreyra Cecilia, Ashley Elizabeth A, Simpson Andrew J H
Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR.
FIND, Geneva, Switzerland.
JAC Antimicrob Resist. 2022 Feb 7;4(1):dlac006. doi: 10.1093/jacamr/dlac006. eCollection 2022 Mar.
There is a need for simple microbiology diagnostics to enable antimicrobial resistance surveillance in low- and middle-income countries.
To investigate the field utility of InTray COLOREX plates for urine culture and ESBL detection.
Clinical urine samples from Mahosot Hospital, Vientiane, Lao PDR were inoculated onto chromogenic media and InTray COLOREX Screen plates between June and August 2020. Urine and isolates from other clinical specimens were inoculated onto COLOREX ESBL plates. A simulated field study investigating the field utility of the InTray COLOREX plates was also completed.
In total, 355 urine samples were inoculated onto standard chromogenic agar and InTray COLOREX Screen plates, and 154 urine samples and 54 isolates from other clinical specimens on the COLOREX ESBL plates. Growth was similar for the two methods (COLOREX Screen 41%, standard method 38%) with 20% discordant results, mainly due to differences in colony counts or colonial appearance. Contamination occurred in 13% of samples, with the COLOREX Screen plates showing increased contamination rates, potentially due to condensation. ESBL producers were confirmed from 80% of isolates from the COLOREX ESBL plates, and direct plating provided rapid detection of presumptive ESBL producers. also grew well on the ESBL plates, a relevant finding in this melioidosis-endemic area.
The InTray COLOREX Screen and ESBL plates were simple to use and interpret, permitting rapid detection of uropathogens and ESBLs, and have the potential for easy transport and storage from field sites and use in laboratories with low capacity.
在低收入和中等收入国家，需要简单的微生物学诊断方法来进行抗菌药物耐药性监测。
研究InTray COLOREX平板用于尿培养和超广谱β-内酰胺酶（ESBL）检测的现场实用性。
2020年6月至8月期间，将老挝万象玛霍索医院的临床尿样接种到显色培养基和InTray COLOREX筛查平板上。将尿样和来自其他临床标本的分离株接种到COLOREX ESBL平板上。还完成了一项调查InTray COLOREX平板现场实用性的模拟现场研究。
总共355份尿样接种到标准显色琼脂和InTray COLOREX筛查平板上，154份尿样以及来自其他临床标本的54株分离株接种到COLOREX ESBL平板上。两种方法的生长情况相似（COLOREX筛查法为41%，标准方法为38%），有20%的结果不一致，主要是由于菌落计数或菌落外观的差异。13%的样本出现污染，COLOREX筛查平板的污染率有所增加，可能是由于冷凝所致。从COLOREX ESBL平板上80%的分离株中确认了ESBL产生菌，直接接种可快速检测出推定的ESBL产生菌。 在ESBL平板上也生长良好，这在这个类鼻疽流行地区是一个相关发现。
InTray COLOREX筛查平板和ESBL平板使用和解读简单，能够快速检测尿路病原体和ESBL，并且有可能便于从现场运输和储存，并在低能力实验室中使用。
