Anderson R L, Highsmith A K, Holland B W
J Clin Microbiol. 1986 Mar;23(3):465-8. doi: 10.1128/jcm.23.3.465-468.1986.
The intrinsic and extrinsic microbial contamination of large-volume parenterals has been associated with bacteremias in hospitalized patients. When epidemiologic data suggest the association of contaminated intravenous (i.v.) fluids with disease, appropriate laboratory methods must be devised to analyze quickly the suspect fluid. A study was undertaken to compare three laboratory test methods (standard pour plate [SPP] technique, ATP procedure, and Limulus amebocyte lysate [LAL] assay) for detecting the presence of microorganisms in artificially contaminated i.v. fluid. SPP proved the most sensitive of the assay techniques used, but a 24-h period was required for microbial growth. The LAL assay appeared more sensitive in detecting i.v. contamination than the ATP method. These studies suggest that the LAL and ATP methods can be used for the rapid detection of microbial contamination in i.v. fluid. SPP and LAL procedures would have practical laboratory application when the contamination of i.v. fluids is suspected, and they are more sensitive in detecting microbial contamination in these solutions than the ATP method.
大容量注射剂的内源性和外源性微生物污染与住院患者的菌血症有关。当流行病学数据表明受污染的静脉输液与疾病有关时，必须设计适当的实验室方法来快速分析可疑液体。进行了一项研究，比较三种实验室检测方法（标准倾注平板[SPP]技术、ATP程序和鲎试剂[LAL]测定法）用于检测人工污染的静脉输液中微生物的存在情况。在所用的检测技术中，SPP被证明是最灵敏的，但微生物生长需要24小时。LAL测定法在检测静脉输液污染方面似乎比ATP方法更灵敏。这些研究表明，LAL和ATP方法可用于快速检测静脉输液中的微生物污染。当怀疑静脉输液受到污染时，SPP和LAL程序在实验室中有实际应用价值，并且它们在检测这些溶液中的微生物污染方面比ATP方法更灵敏。
