利用鲎试剂体外内毒素测定法快速检测受污染的静脉输液。
Rapid detection of contaminated intravenous fluids using the Limulus in vitro endotoxin assay.
作者信息
Jorgensen J H, Smith R F
出版信息
Appl Microbiol. 1973 Oct;26(4):521-4. doi: 10.1128/am.26.4.521-524.1973.
Abstract
Intravenous fluids and administration sets may become contaminated with gram-negative bacteria during use and result in a life-threatening situation to the patient. The Limulus in vitro assay for endotoxin was used in two patients whose parenteral fluids had become contaminated with Pseudomonas aeruginosa. This test allowed rapid detection of the contaminated intravenous fluids and demonstrated a concomitant endotoxemia in both patients. The same strains of pseudomon were subsequently cultured from each patient's blood, intravenous catheter tip, and parenteral fluid and administration set. A different serotype of pseudomonas was unique to each patient, indicating two separate and unrelated cases of accidental contamination of the administration sets. Endotoxin-like activity was also demonstrated from several brands of commercial human serum albumin, which may contribute low-level activity detectable by the Limulus assay.
摘要
静脉输液和输液器在使用过程中可能被革兰氏阴性菌污染，从而给患者带来危及生命的情况。对两名患者使用了鲎试剂体外内毒素检测法，他们的胃肠外营养液已被铜绿假单胞菌污染。该检测方法能够快速检测出被污染的静脉输液，并证实两名患者均伴有内毒素血症。随后从每位患者的血液、静脉导管尖端、胃肠外营养液及输液器中培养出相同菌株的假单胞菌。每位患者的假单胞菌血清型不同，表明输液器意外污染为两起独立且不相关的事件。几种市售人血清白蛋白品牌也显示出内毒素样活性，这可能是鲎试剂检测法可检测到的低水平活性的原因。
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