Terranova V P, DiFlorio R, Hujanen E S, Lyall R M, Liotta L A, Thorgeirsson U, Siegal G P, Schiffmann E
J Clin Invest. 1986 Apr;77(4):1180-6. doi: 10.1172/JCI112419.
Polymorphonuclear neutrophils (PMN) traverse basement membrane to reach sites of infection. We have studied the role of laminin, a specific basement membrane component, in this process using three assay systems. In the Boyden chamber, laminin was found to stimulate chemotaxis of neutrophils while fibronectin did not. Co-incubation of cells with antibody to laminin blocked this chemotaxis, while antibody to fibronectin was without effect. In the human amnion system, neutrophils were shown to penetrate through the tissue when the peptide chemoattractant f-Met-Leu-Phe was placed on the opposing side. Antibody to laminin, but not to fibronectin, blocked this penetration. In an attachment assay system, laminin, but not fibronectin, was found to increase dispase-treated neutrophil attachment to type IV (basement membrane) collagen-coated plastic and to a plastic substrate itself. Electrophoretic analysis of PMN extract indicated the presence of laminin, and indirect immunofluorescence suggested that laminin is localized on the surface of the neutrophils. These data suggest that PMN can bind laminin on their cell surfaces, use laminin to attach to basement (type IV) membrane collagen, and migrate toward a gradient of laminin. These properties may be important for the passage of neutrophils from the circulation to sites of infection.
多形核中性粒细胞（PMN）穿过基底膜到达感染部位。我们使用三种检测系统研究了层粘连蛋白（一种特定的基底膜成分）在此过程中的作用。在博伊登小室中，发现层粘连蛋白可刺激中性粒细胞的趋化作用，而纤连蛋白则无此作用。将细胞与抗层粘连蛋白抗体共同孵育可阻断这种趋化作用，而抗纤连蛋白抗体则无效。在人羊膜系统中，当将肽趋化剂f-Met-Leu-Phe置于相对侧时，中性粒细胞可穿透该组织。抗层粘连蛋白抗体而非抗纤连蛋白抗体可阻断这种穿透。在附着检测系统中，发现层粘连蛋白而非纤连蛋白可增加经分散酶处理的中性粒细胞与IV型（基底膜）胶原包被的塑料以及塑料底物本身的附着。对PMN提取物的电泳分析表明存在层粘连蛋白，间接免疫荧光表明层粘连蛋白定位于中性粒细胞表面。这些数据表明，PMN可在其细胞表面结合层粘连蛋白，利用层粘连蛋白附着于基底（IV型）膜胶原，并朝着层粘连蛋白梯度迁移。这些特性对于中性粒细胞从循环系统转移至感染部位可能很重要。
