基于 PET 的荧光探针用于监测巨噬细胞激活和铁死亡中的不稳定 Fe(II)池。
A PET-based fluorescent probe for monitoring labile Fe(II) pools in macrophage activations and ferroptosis.
机构信息
State Key Laboratory of Bioengineering Reactor, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, Shanghai Key Laboratory of New Drug Design, and Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science & Technology, 130 Meilong Road, Shanghai 200237, China.
Fluorescence Research Group, Singapore University of Technology and Design, 8 Somapah Road, Singapore 487372, Singapore.
出版信息
Chem Commun (Camb). 2022 Mar 1;58(18):2979-2982. doi: 10.1039/d1cc06611k.
A fluorescent probe (COU-LIP-1) for monitoring labile Fe(II) pools (LIP) with high selectivity and sensitivity was developed utilizing coumarin 343 as the fluorophore and 3-nitrophenylazanyl ester as both the reactive group and the fluorescence quenching group. Fe(II)-induced reductive cleavage of the N-O bond results in a turn-on response a photo-induced photon transfer (PET) mechanism. The probe was applied for monitoring labile iron(II) changes in M1 and M2a macrophage activations and also erastin-induced ferroptosis, providing a powerful tool for selectively sensing LIP under both physiological and stressed conditions.
开发了一种利用香豆素 343 作为荧光团，3-硝基苯叠氮酯作为反应基团和荧光猝灭基团的荧光探针（COU-LIP-1），用于高选择性和高灵敏度地监测易变铁(II)池（LIP）。Fe(II)诱导的 N-O 键还原裂解导致开启响应-一种光诱导电子转移（PET）机制。该探针用于监测 M1 和 M2a 巨噬细胞激活过程中易变铁(II)的变化，以及依拉司琼诱导的铁死亡，为在生理和应激条件下选择性检测 LIP 提供了有力工具。
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