Friedrich-Loeffler-Institut, Südufer 10, 17943 Greifswald-Insel Riems, Germany.
Central Veterinary Research Laboratory, Dubai P.O. Box 597, United Arab Emirates.
Viruses. 2023 Jun 5;15(6):1325. doi: 10.3390/v15061325.
The peste des petits ruminant (PPR) virus is a transboundary virus found in small domestic ruminants that causes high morbidity and mortality in naive herds. PPR can be effectively controlled and eradicated by vaccinating small domestic ruminants with a live-attenuated peste des petits ruminant virus (PPRV) vaccine, which provides long-lasting immunity. We studied the potency and safety of a live-attenuated vaccine in goats by detecting their cellular and humoral immune responses. Six goats were subcutaneously vaccinated with a live-attenuated PPRV vaccine according to the manufacturer's instructions, and two goats were kept in contact. Following vaccination, the goats were monitored daily, and we recorded their body temperature and clinical score. Heparinized blood and serum were collected for a serological analysis, and swab samples and EDTA blood were collected to detect the PPRV genome. The safety of the used PPRV vaccine was confirmed by the absence of PPR-related clinical signs, a negative pen-side test, a low virus genome load as detected with RT-qPCR on the vaccinated goats, and the lack horizontal transmission between the in-contact goats. The strong humoral and cellular immune responses detected in the vaccinated goats showed that the live-attenuated PPRV vaccine has a strong potency in goats. Therefore, live-attenuated vaccines against PPR can be used to control and eradicate PRR.
小反刍兽疫病毒（PPRV）是一种在小反刍动物中发现的跨界病毒，在无免疫力的畜群中会导致高发病率和死亡率。通过给小反刍动物接种弱毒活疫苗，可以有效控制和消灭小反刍兽疫，该疫苗能提供持久的免疫力。我们通过检测细胞和体液免疫应答来研究弱毒活疫苗在山羊中的效力和安全性。按照制造商的说明，6 只山羊通过皮下接种弱毒活 PPRV 疫苗，2 只山羊保持接触。接种后，每天监测山羊，记录体温和临床评分。采集肝素化血液和血清进行血清学分析，采集拭子样本和 EDTA 血液以检测 PPRV 基因组。使用的 PPRV 疫苗安全性通过以下几点得到确认：没有与 PPR 相关的临床症状、笔侧试验阴性、接种山羊的 RT-qPCR 检测到的病毒基因组载量低，以及接触山羊之间没有水平传播。在接种疫苗的山羊中检测到的强烈的体液和细胞免疫应答表明，弱毒活 PPRV 疫苗在山羊中具有很强的效力。因此，弱毒活疫苗可用于控制和消灭小反刍兽疫。
