Section on Translational Tumor Immunology, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Building 10, Room 7N240C, Bethesda, MD, 20892, USA.
Rutgers Cancer Center at Rutgers, The State University of New Jersey, New Brunswick, NJ, USA.
BMC Immunol. 2022 Feb 11;23(1):5. doi: 10.1186/s12865-022-00478-4.
Determining T cell responses to naturally processed and presented antigens is a critical immune correlate to determine efficacy of an investigational immunotherapeutic in clinical trials. In most cases, minimal epitopes and HLA restriction elements are unknown.
Here, we detail the experimental use of ex vivo expanded autologous B cells as antigen presenting cells to overcome the limitation of unknown HLA restriction, and the use of electroporated full length mRNA encoding full length parental proteins to ensure that any observed T cell responses are specific for antigens that are naturally processed and presented.
This technique can serve as useful experimental approach to determine the induction or enhancement of specific responses to naturally processed and presented antigens on HLA class I molecules in peripheral blood or tumor infiltrating T cells.
确定 T 细胞对天然加工和呈递抗原的反应是确定临床试验中研究性免疫治疗疗效的关键免疫相关性。在大多数情况下，最小表位和 HLA 限制因素是未知的。
在这里，我们详细介绍了使用体外扩增的自体 B 细胞作为抗原呈递细胞来克服 HLA 限制未知的局限性的实验用途，以及使用电穿孔全长 mRNA 编码全长亲本蛋白的方法，以确保观察到的 T 细胞反应是针对天然加工和呈递的抗原的特异性反应。
该技术可作为一种有用的实验方法，用于确定在周围血或肿瘤浸润 T 细胞中 HLA Ⅰ类分子上天然加工和呈递抗原的特异性反应的诱导或增强。
