Department of Food Science and Technology, College of Agriculture and Life Sciences, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do, 54896, Republic of Korea; Department of Agricultural Convergence Technology, College of Agriculture and Life Science, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do, 54896, Republic of Korea.
Department of Bionanotechnology and Bioconvergence Engineering, Graduate School, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do, 54896, Republic of Korea; Department of Bionanosystem Engineering, Graduate School, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do, 54896, Republic of Korea.
Environ Res. 2022 Jul;210:112864. doi: 10.1016/j.envres.2022.112864. Epub 2022 Feb 8.
This study was aimed on the eco-friendly synthesis of silver nanoparticles (AgNPs), reduced graphene oxide (rGO) and AgNPs decorated rGO (rGO/AgNPs) nanocomposite and appraisal of their bioactivities and toxicity. As-prepared nanomaterials were established through high resolution X-ray diffraction (HR-XRD), high resolution transmission electron microscopy (HR-TEM), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy, UV-Vis. spectroscopy and Fourier transform infrared spectroscopy (FT-IR). In this study, leaves extract, graphene oxide (GO) and rGO did not show antibacterial and anticancer activities; no significant embryo toxicity was recorded. On the other hand, AgNPs displayed good antibacterial and anticancer activities; however, higher toxic effects were observed even at the lowest test concentration (0.7 μg/ml). In case of rGO/AgNPs nanocomposite, significant antibacterial activity together with low cytotoxicity was noticed. Interestingly, the embryo toxicity of AgNPs was significantly reduced by rGO, implying the biocompatible nature of as-synthesized nanocomposite. Taken together, these results clearly suggest that rGO/AgNPs nano hybrid composite could be developed as the promising biomaterial for future biomedical applications.
本研究旨在环保合成银纳米粒子（AgNPs）、还原氧化石墨烯（rGO）和 AgNPs 修饰的 rGO（rGO/AgNPs）纳米复合材料，并评估它们的生物活性和毒性。通过高分辨率 X 射线衍射（HR-XRD）、高分辨率透射电子显微镜（HR-TEM）、X 射线光电子能谱（XPS）、拉曼光谱、紫外可见分光光度计（UV-Vis）和傅里叶变换红外光谱（FT-IR）对所制备的纳米材料进行了表征。在本研究中，叶提取物、氧化石墨烯（GO）和 rGO 没有表现出抗菌和抗癌活性；没有记录到明显的胚胎毒性。另一方面，AgNPs 表现出良好的抗菌和抗癌活性；然而，即使在最低测试浓度（0.7μg/ml）下，也观察到了更高的毒性作用。对于 rGO/AgNPs 纳米复合材料，观察到了显著的抗菌活性和低细胞毒性。有趣的是，rGO 显著降低了 AgNPs 的胚胎毒性，这表明所合成的纳米复合材料具有生物相容性。综上所述，这些结果清楚地表明，rGO/AgNPs 纳米杂化复合材料可以作为有前途的生物材料，用于未来的生物医学应用。
