Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul 05029, Korea.
Int J Mol Sci. 2017 Nov 28;18(12):2549. doi: 10.3390/ijms18122549.
Recently, graphene and graphene related nanocomposite receive much attention due to high surface-to-volume ratio, and unique physiochemical and biological properties. The combination of metallic nanoparticles with graphene-based materials offers a promising method to fabricate novel graphene-silver hybrid nanomaterials with unique functions in biomedical nanotechnology, and nanomedicine. Therefore, this study was designed to prepare graphene oxide (GO) silver nanoparticles (AgNPs) nanocomposite (GO-AgNPs) containing two different nanomaterials in single platform with distinctive properties using luciferin as reducing agents. In addition, we investigated the effect of GO-AgNPs on differentiation in SH-SY5Y cells. The synthesized GO-AgNPs were characterized by ultraviolet-visible absorption spectroscopy (UV-vis), X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Raman spectroscopy. The differentiation was confirmed by series of cellular and biochemical assays. The AgNPs were distributed uniformly on the surface of graphene oxide with an average size of 25 nm. As prepared GO-AgNPOs induces differentiation by increasing the expression of neuronal differentiation markers and decreasing the expression of stem cell markers. The results indicated that the redox biology involved the expression of various signaling molecules, which play an important role in differentiation. This study suggests that GO-AgNP nanocomposite could stimulate differentiation of SH-SY5Y cells. Furthermore, understanding the mechanisms of differentiation of neuroblastoma cells could provide new strategies for cancer and stem cell therapies. Therefore, these studies suggest that GO-AgNPs could target specific chemotherapy-resistant cells within a tumor.
最近，由于具有高的比表面积和独特的物理化学和生物学性质，石墨烯和石墨烯相关纳米复合材料受到了广泛关注。将金属纳米粒子与基于石墨烯的材料结合起来，为制备具有独特功能的新型石墨烯-银杂化纳米材料提供了一种很有前途的方法，这种材料在生物医学纳米技术和纳米医学中有广泛的应用。因此，本研究旨在使用荧光素作为还原剂，在单个平台上制备含有两种不同纳米材料的氧化石墨烯（GO）-银纳米粒子（AgNPs）纳米复合材料（GO-AgNPs），这两种纳米材料具有独特的性质。此外，我们研究了 GO-AgNPs 对 SH-SY5Y 细胞分化的影响。通过紫外-可见吸收光谱（UV-vis）、X 射线衍射（XRD）、扫描电子显微镜（SEM）、透射电子显微镜（TEM）和拉曼光谱对合成的 GO-AgNPs 进行了表征。通过一系列细胞和生化分析来确认分化。AgNPs 均匀分布在氧化石墨烯的表面上，平均粒径为 25nm。所制备的 GO-AgNPOs 通过增加神经元分化标志物的表达和降低干细胞标志物的表达来诱导分化。结果表明，氧化还原生物学涉及到各种信号分子的表达，这些信号分子在分化中起着重要作用。本研究表明，GO-AgNP 纳米复合材料可以刺激 SH-SY5Y 细胞的分化。此外，了解神经母细胞瘤细胞分化的机制可为癌症和干细胞治疗提供新的策略。因此，这些研究表明，GO-AgNPs 可以靶向肿瘤内特定的化疗耐药细胞。
