Wu You, Chen Shoucheng, Luo Pu, Deng Shudan, Shan Zhengjie, Fang Jinghan, Liu Xingchen, Xie Jiaxin, Liu Runheng, Wu Shiyu, Wu Xiayi, Chen Zetao, Yeung Kelvin W K, Liu Quan, Chen Zhuofan
Hospital of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangdong Research Center for Dental and Cranial Rehabilitation and Material Engineering, Guangzhou, China.
Department of Orthopaedics and Traumatology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China; Shenzhen Key Laboratory for Innovative Technology in Orthopaedic Trauma, The University of Hong Kong-Shenzhen Hospital, Shenzhen, China.
Acta Biomater. 2022 Apr 15;143:159-172. doi: 10.1016/j.actbio.2022.02.004. Epub 2022 Feb 9.
Biogenic collagen membranes have been widely used as soft tissue barriers in guided bone regeneration (GBR) and guided tissue regeneration (GTR). Nevertheless, their clinical performance remains unsatisfactory because of their low mechanical strength and fast degradation rate in vivo. Although cross-linking with chemical agents is effective and reliable for prolonging the degradation time of collagen membranes, some adverse effects including potential cytotoxicity and undesirable tissue integration have been observed during this process. As a fundamental nutritional trace element, zinc plays an active role in promoting the growth of cells and regulating the degradation of the collagen matrix. Herein, a biogenic collagen membrane was cross-linked with glutaraldehyde-alendronate to prolong its degradation time. The physiochemical and biological properties were enhanced by the incorporation of zinc-doped nanohydroxyapatite (nZnHA), with the native structure of collagen preserved. Specifically, the cross-linking combined with the incorporation of 1% and 2% nZnHA seemed to endow the membrane with the most appropriate biocompatibility and tissue integration capability among the cross-linked membranes, as well as offering a degradation period of six weeks in a rat subcutaneous model. Thus, improving the clinical performance of biogenic collagen membranes by cross-linking together with the incorporation of nZnHA is a promising strategy for the improvement of biogenic collagen membranes. STATEMENT OF SIGNIFICANCE: The significance of this research includes.
生物源性胶原膜已被广泛用作引导骨再生（GBR）和引导组织再生（GTR）中的软组织屏障。然而，由于其机械强度低和体内降解速度快，其临床性能仍不尽人意。尽管与化学试剂交联对于延长胶原膜的降解时间是有效且可靠的，但在此过程中已观察到一些不良反应，包括潜在的细胞毒性和不良的组织整合。作为一种基本的营养微量元素锌，在促进细胞生长和调节胶原基质降解方面发挥着积极作用。在此，将生物源性胶原膜与戊二醛 - 阿仑膦酸盐交联以延长其降解时间。通过掺入锌掺杂的纳米羟基磷灰石（nZnHA）增强了其物理化学和生物学特性，同时保留了胶原的天然结构。具体而言，交联结合掺入1%和2%的nZnHA似乎使该膜在交联膜中具有最合适的生物相容性和组织整合能力，并且在大鼠皮下模型中提供了六周的降解期。因此，通过交联并掺入nZnHA来改善生物源性胶原膜的临床性能是一种改善生物源性胶原膜的有前景的策略。重要性声明：本研究的重要性包括。
